ELISA Kits
Enzyme-linked Immunosorbent Assay (ELISA) kit is immunochemical method for the detection of substances such as antibodies and viral antigens, or other proteins or peptides, using the immuno binding reaction between antigens and antibodies. We supply 7 series of ELISA Test Kits, used to test infectious diseases, including Hepatitis, Tuberculosis, Acquired Immune Deficiency Syndrome (AIDS) and Syphilis.
Components of a typical ELISA Kit
MICROWELL PLATE: 1 plate
Blank micro-well strips fixed on white strip holder. The plate is sealed in aluminum pouch with desiccant. 8×12/12×8-well strips per plate. Each well contains recombinant HCV antigens. The micro-well strips can be broken to be used separately. Place unused wells or strips in the plastic sealable storage bag together with the desiccant and return to 2~8 °C.
NEGATIVE CONTROL: 1 vial
Blue-colored liquid filled in a vial with green screw cap. 0.2ml per vial. Protein-stabilized buffer tested non-reactive for HCV antibodies. Preservatives: 0.1% ProClin 300. Ready to use as supplied. Once open, stable for one month at 2-8 °C.
POSITIVE CONTROL SERUM: 1 vial
Red-colored liquid filled in a vial with red screw cap. 0.2ml per vial. anti-HCV antibodies diluted in protein-stabilized buffer Preservatives: 0.1% ProClin 300. Ready to use as supplied. Once open, stable for one month at 2-8 °C.
SPECIMEN DILUENT: 1 vial
Blue liquid filled in a white vial with blue screw cap. 13ml per vial. Protein-stabilized buffer, casein, and sucrose solution. Ready to use as supplied. Once open, stable for one month at 2-8 °C.
HRP-CONJUGATE REAGENT: 1 vial
Red-colored liquid filled in a white vial with red screw cap. 13ml per vial. Horseradish peroxidase-conjugated rabbit anti-human IgG antibodies. Ready to use as supplied. Once open, stable for one month at 2-8 °C.
WASH BUFFER: 1 bottle
DILUTE BEFORE USE: Colorless liquid filled in a clear bottle with white screw cap. 50ml per bottle. PH 7.4, 20 × PBS (Contains Tween-20 as a detergent) The concentrate must be diluted 1 to 20 with distilled/deionized water before use. Once diluted, stable for one week at room temperature, or for two weeks at 2-8 °C.
CHROMOGEN SOLUTION A: 1 vial
Colorless liquid filled in a white vial with green screw cap. 8ml per vial. Urea peroxide solution. Ready to use as supplied. Once open, stable for one month at 2-8 °C.
CHROMOGEN SOLUTION B: 1 vial
Colorless liquid filled in a black vial with black screw cap. 8ml per vial. TMB solution(Tetramethyl benzidine dissolved in citric acid). Ready to use as supplied. Once open, stable for one month at 2-8 °C.
STOP SOLUTION: 1 vial
Colorless liquid filled in a white vial with yellow screw cap. 8ml per vial. Diluted sulfuric acid solution (2.0M H2SO4). Ready to use as supplied.
PLASTIC SEALABLE BAG: 1 unit
For enclosing the strips not in use.
CARDBOARD PLATE COVER: 2 sheets
To cover the plates during incubation and prevent evaporation or contamination of the wells.
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