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HAV IgM and IgG ELISA Kits

HAV IgM ELISA kit and IgG ELISA kit are intended to be used to massively screen human blood specimens for the detection of HAV specific IgM and IgG antibodies in human serum specimens.

HAV IgM ELISA Kit

HAV Antibodies ELISA Kits

HAV IgM and HAV IgG ELISA kits are enzyme-linked immunosorbent assay kits, used to detect IgM and IgG antibodies specific to Hepatitis A infection in human serum specimen, which can be used in mass blood screening settings, such as hospital laboratories, blood banks; and also can be used as an aid in the diagnosis and administration of Hepatitis A disease.

HAV IgG & IgM ELISA Principles

HAV-IgG ELISA kit is based on solid phase, one-step incubation competitive principle ELISA method; while HAV IgM ELISA kit is based on solid phase, two-step incubation, antibody capture ELISA assay method.

If the target antibodies present in the specimen, after the incubation, washing, color development and stopping, yellow color will develop on the polystyrene microwell strip; The amount of color intensity can be measured and is proportional to the amount of antibody captured in the wells, and to the sample respectively. Wells containing samples negative for the target HAV-IgM or IgG antibodies remain colorless.

Clinical Value of HAV IgG and IgM Differentiation

Hepatitis A is a self-limited disease and chronic stage or other complications are rare. Infections occur early in life in areas where sanitation is poor and living conditions are crowded.

The infection with HAV induces strong immunological response and elevated levels first of IgM and then IgG are detectable within a few days after the onset of the symptoms. The presence of antiā€“HAV IgM is an important serological marker for early detection and observation of the clinical manifestation of the disease.

Detection of HAV IgG in the absence of HAV IgM, indicates either a previous infection with HAV or a successful vaccination. A titer of 10 IU/L HAV IGG is considered to be the minimal concentration of a valid immunization.

Add Hepatitis A ELISA Kits to Inquiry Cart How to Use

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
HAV 2614 HAV IGM ELISA Kit serum, plasma96 Boxed: 40 Kits
HAV 2624 HAV IGG ELISA Kit serum, plasma96 Boxed: 40 Kits

HAV IGM ELISA Kit

General Information

Catalog No.:HAV 2614
Description:HAV IGM ELISA Kit
Category:Hepatitis Disease
Style:Microwell
Unit:Box
Specimen:serum, plasma
No. of Step:Two Step
Reading Time:20-40-15 Minutes
Cut Off:
Specification:48T/96T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:Capture ELISA

Packing Specification

Tests per Kit:96 Tests
Box Dimension:13.5 * 9.0 * 8.0 CM
Kits per CTN:40 Kits
Carton Dimension:59 * 45 * 38 CM
Tests per CTN (BULK):40 Tests
Carton Volume:0.100 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided

  • MICROWELL PLATE: 1 plate;
  • NEGATIVE CONTROL: 1 vial;
  • POSITIVE CONTROL SERUM: 1 vial;
  • SPECIMEN DILUENT: 1 vial;
  • HRP-CONJUGATE REAGENT: 1 vial;
  • WASH BUFFER: 1 bottle;
  • CHROMOGEN SOLUTION A: 1 vial;
  • CHROMOGEN SOLUTION B: 1 vial;
  • STOP SOLUTION: 1 vial;
  • PLASTIC SEALABLE BAG: 1 piece;
  • CARDBOARD PLATE COVER;
  • PACKAGE INSERTS: 1 copy.

Specimen

Plasma Collection

Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.

Separate the plasma by centrifugation.

Carefully withdraw the plasma into a new pre-labeled tube.

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum, plasma Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Capture ELISA

ELISA Capture Method Illustration

For the detection of IgM-class antibodies, in order to avoid the interference from the IgG class, a solid phase, two-step incubation, antibody capture assays is usually used.

In capture ELISA kit, polystyrene microwell strips are pre-coated with antibodies directed to human IgM (anti-i chain). The patient’s serum/plasma sample is added, and during the first incubation, any IgM antibodies will be captured in the wells, and IgG antibodies on the other hand will not.

After washing out all other components of the sample and in particular IgG antibodies, the target IgM antibodies captured on the solid phase are detected by the addition of viral antigens conjugated to horseradish peroxidase (HRP). During the second incubation, the conjugated antigens will specifically react with the viral specific IgM antibodies.

After washing to remove unbound conjugates, Chromogen solutions are added to the wells. During the third incubation, the colorless chromogens are hydrolyzed by the bound HRP conjugate to a blue colored product. The blue color turns yellow after stopping the reaction with sulfuric acid, indicating a positive result; the amount of color can be measured and is proportional to the amount of antibody in the sample.

Wells containing samples negative for the target IgM antibodies remain colorless, indicating negative result.

Information | Packing | Specimen | Principle

HAV IGG ELISA Kit

General Information

Catalog No.:HAV 2624
Description:HAV IGG ELISA Kit
Category:Hepatitis Disease
Style:Microwell
Unit:Box
Specimen:serum, plasma
No. of Step:One Step
Reading Time:60-15 Minutes
Cut Off:
Specification:48T/96T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:Competitive Binding ELISA

Packing Specification

Tests per Kit:96 Tests
Box Dimension:13.5 * 9.0 * 8.0 CM
Kits per CTN:40 Kits
Carton Dimension:59 * 45 * 38 CM
Tests per CTN (BULK):40 Tests
Carton Volume:0.100 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided

  • MICROWELL PLATE: 1 plate;
  • NEGATIVE CONTROL: 1 vial;
  • POSITIVE CONTROL SERUM: 1 vial;
  • SPECIMEN DILUENT: 1 vial;
  • HRP-CONJUGATE REAGENT: 1 vial;
  • WASH BUFFER: 1 bottle;
  • CHROMOGEN SOLUTION A: 1 vial;
  • CHROMOGEN SOLUTION B: 1 vial;
  • STOP SOLUTION: 1 vial;
  • PLASTIC SEALABLE BAG: 1 piece;
  • CARDBOARD PLATE COVER;
  • PACKAGE INSERTS: 1 copy.

Specimen

Plasma Collection

Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.

Separate the plasma by centrifugation.

Carefully withdraw the plasma into a new pre-labeled tube.

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum, plasma Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Competitive Binding ELISA

ELISA Competitive Method Illustration

ELISA kit based on competitive principle is one-step test kit, in which a fixed amount of purified viral specific antigens is pre-coated in the microwell strips.

During the testing process, the sample and monoclonal antibodies conjugated to horseradish peroxidase (HRP) is added to the microwells together. During incubation, the target antibodies, if present in the sample, compete with the HRP conjugated antibodies to bind to the pre-coated antigens. When no target antibodies present in the sample, the HRP labeled antibodies will bind with the antigens inside the wells and any unbound HRP-Conjugate is removed during washing.

Chromogen A and B solutions are added into the wells and during the second incubation, the colorless Chromogens are hydrolyzed by the bound HRP-Conjugate to a blue colored product. The blue color turns yellow after stopping the reaction with sulfuric acid, indicating a NEGATIVE result.

No or low color developing suggests the presence of the target antibodies in the sample. So the color developed on the microwell strips is negatively related to the concentration of the target antibodies.

Information | Packing | Specimen | Principle