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HCV Antibody ELISA Kit

Anti-HCV ELISA kit is intended to be used to detect HCV specific antibodies in human serum specimen, as an aid in the diagnosis of Hepatitis-C infection.

HCV Antibody ELISA Kit

Anti-HCV ELISA Kit

This HCV antibody ELISA kit is an enzyme-linked immunosorbent assay, for the qualitative detection of antibodies to hepatitis C virus in human serum or plasma. Anti-HCV ELISA kit is intended for screening blood donors at blood banks, and diagnosing patients related to infection with hepatitis C virus.

HCV Antibody ELISA Kit, 3rd Generation

Since the introduction in 1990 of anti-HCV screening of blood donations, the incidence of this infection in transfusion recipients has been significantly reduced. The first generation of HCV ELISAs showed limited sensitivity and specificity and was produced using recombinant proteins complementary to the NS4 (c100-3) region of the HCV genome as antigens. Second generation tests, which included recombinant / synthetic antigens from the Core (c22) and nonstructural regions NS3 (c33c, c100-3) and NS4 (c100-3, c200) resulted in a remarked improvement in sensitivity and specificity.

Clinical studies show that significant amount of HCV infected individuals develop antibodies to NS5 non-structural protein of the virus. For this, the third generation Anti-HCV ELISA kits include antigens from the NS5 region of the viral genome in addition to NS3 (c200), NS4 (c200) and the Core (c22). Third generation of anti-HCV EIA kits have improved sensitivity and shorten the time between infection with HCV and the appearance of detectable antibodies (window period) to 60 days.

Why Anti-HCV Screening

Hepatitis C virus (HCV) is an envelope, single stranded positive sense RNA (9.5 kb) virus belonging to the family of Flaviviridae. Six major genotypes and series of subtypes of HCV have been identified. Isolated in 1989, HCV is now recognized as the major cause for transfusion associated non-A, non-B hepatitis. Since the introduction of Anti-HCV EIA kit in 1990 for the anti-HCV screening of blood donations, the incidence of this infection in transfusion recipients has been significantly reduced.

Add Hepatitis C ELISA Kits to Inquiry Cart How to Use

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
HCV 2714 Anti-HCV ELISA Kit serum, plasma96 Boxed: 40 Kits

Anti-HCV ELISA Kit

General Information

Catalog No.:HCV 2714
Description:Anti-HCV ELISA Kit
Category:Hepatitis Disease
Style:Microwell
Unit:Box
Specimen:serum, plasma
No. of Step:Two Step
Reading Time:30-30-15 Minutes
Cut Off:
Specification:48T/96T/480T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:Indirect ELISA

Packing Specification

Tests per Kit:96 Tests
Box Dimension:13.5 * 9.0 * 8.0 CM
Kits per CTN:40 Kits
Carton Dimension:59 * 45 * 38 CM
Tests per CTN (BULK):40 Tests
Carton Volume:0.100 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided

  • MICROWELL PLATE: 1 plate;
  • NEGATIVE CONTROL: 1 vial;
  • POSITIVE CONTROL SERUM: 1 vial;
  • SPECIMEN DILUENT: 1 vial;
  • HRP-CONJUGATE REAGENT: 1 vial;
  • WASH BUFFER: 1 bottle;
  • CHROMOGEN SOLUTION A: 1 vial;
  • CHROMOGEN SOLUTION B: 1 vial;
  • STOP SOLUTION: 1 vial;
  • PLASTIC SEALABLE BAG: 1 piece;
  • CARDBOARD PLATE COVER;
  • PACKAGE INSERTS: 1 copy.

Specimen

Plasma Collection

Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.

Separate the plasma by centrifugation.

Carefully withdraw the plasma into a new pre-labeled tube.

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum, plasma Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Indirect ELISA

ELISA Indirect Method Illustration

With indirect ELISA kit, polystyrene microwell strips are pre-coated with purified or recombinant viral antigens.

The antibody detection is achieved in two steps. During the first incubation step, the target antibodies, if present, will be bound to the solid phase pre-coated antigens.

The wells are washed to remove unbound serum proteins, and rabbit anti-human IgG antibodies (anti-IgG) conjugated to horseradish peroxidase (HRP) are added. During the second incubation step, these HRP-conjugated antibodies will be bound to any antigen-antibody complexes previously formed.

Then the unbound HRP-conjugate is then removed by washing. Chromogen solutions containing Tetramethyl benzidine (TMB) and urea peroxide are added to the wells. In presence of the antigen-antibody-anti-IgG (HRP) immuno complex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue colored product which turns blue color turns yellow after stopping the reaction with sulfuric acid, indicating a positive result.

Wells containing samples negative for the target antibodies remain colorless.

Information | Packing | Specimen | Principle