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HEV ELISA Kits

HEV IgM ELISA, HEV IgG ELISA and HEV antigen ELISA kits are supplied, which are intended to be used to detect IgM, IgG specific to Hepatitis-E virus and HEV antigen respectively in human serum specimen.

HEV ANTIGEN ELISA Kit

HEV ELISA Kits

Atlas Link Technology Co., Ltd supplies several HEV ELISA kits, including HEV IgM ELISA, HEV IgG ELISA and HEV antigen ELISA kits, which are enzyme-linked immunosorbent assay kits, intended to be used to detect HEV IgM antibody, HEV IgG antibody and HEV antigen respectively in human serum specimen, and can be used in mass blood screening settings, such as hospital laboratories, blood banks; and also can be used in the diagnosis of Hepatitis-E virus infection.

HEV EIA Kits: Testing Principles

Different testing principles are adopted in these HEV ELISA kits. The HEV IgM ELISA is a two-step incubation, solid phase antibody capture ELISA assay; the HEV IgG ELISA employs solid phase, indirect ELISA method for detection of IgG-class antibodies to HEV (anti-HEV) in two-step incubation procedure; while the HEV Antigen ELISA kit is a two-step Incubation, Double Antibody "Sandwich" assay.

Hepatitis-E Disease

Hepatitis E virus (HEV) is a non-enveloped, single-stranded RNA virus. Four genotypes of HEV have been reported. HEV is transmitted mainly by fecal contaminated water and food. HEV infections, endemic and frequently epidemic in developing countries, is seen also in developed countries in a sporadic form with or without a history of traveling to endemic area.

With the discovery of conformational epitopes in HEV, HEV serology was further explored and understood. The phenomenon of long-lasting and protective antibodies to HEV was observed which greatly enhance the understanding to the diagnosis, epidemiology, zoonosis-related studies and vaccine development.

Add Hepatitis E ELISA Kits to Inquiry Cart How to Use

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
HEV 2914 HEV IgM ELISA Kit serum, plasma96 Boxed: 40 Kits
HEV 2924 HEV IgG ELISA Kit serum, plasma96 Boxed: 40 Kits
HEV 2934 HEV Antigen ELISA Kit serum, plasma96 Boxed: 40 Kits

HEV IgM ELISA Kit

General Information

Catalog No.:HEV 2914
Description:HEV IgM ELISA Kit
Category:Hepatitis Disease
Style:Microwell
Unit:Box
Specimen:serum, plasma
No. of Step:Two Step
Reading Time:30-30-15 Minutes
Cut Off:
Specification:96T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:Capture ELISA

Packing Specification

Tests per Kit:96 Tests
Box Dimension:13.5 * 9.0 * 8.0 CM
Kits per CTN:40 Kits
Carton Dimension:59 * 45 * 38 CM
Tests per CTN (BULK):40 Tests
Carton Volume:0.100 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided

  • MICROWELL PLATE: 1 plate;
  • NEGATIVE CONTROL: 1 vial;
  • POSITIVE CONTROL SERUM: 1 vial;
  • SPECIMEN DILUENT: 1 vial;
  • HRP-CONJUGATE REAGENT: 1 vial;
  • WASH BUFFER: 1 bottle;
  • CHROMOGEN SOLUTION A: 1 vial;
  • CHROMOGEN SOLUTION B: 1 vial;
  • STOP SOLUTION: 1 vial;
  • PLASTIC SEALABLE BAG: 1 piece;
  • CARDBOARD PLATE COVER;
  • PACKAGE INSERTS: 1 copy.

Specimen

Plasma Collection

Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.

Separate the plasma by centrifugation.

Carefully withdraw the plasma into a new pre-labeled tube.

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum, plasma Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Capture ELISA

ELISA Capture Method Illustration

For the detection of IgM-class antibodies, in order to avoid the interference from the IgG class, a solid phase, two-step incubation, antibody capture assays is usually used.

In capture ELISA kit, polystyrene microwell strips are pre-coated with antibodies directed to human IgM (anti-i chain). The patient’s serum/plasma sample is added, and during the first incubation, any IgM antibodies will be captured in the wells, and IgG antibodies on the other hand will not.

After washing out all other components of the sample and in particular IgG antibodies, the target IgM antibodies captured on the solid phase are detected by the addition of viral antigens conjugated to horseradish peroxidase (HRP). During the second incubation, the conjugated antigens will specifically react with the viral specific IgM antibodies.

After washing to remove unbound conjugates, Chromogen solutions are added to the wells. During the third incubation, the colorless chromogens are hydrolyzed by the bound HRP conjugate to a blue colored product. The blue color turns yellow after stopping the reaction with sulfuric acid, indicating a positive result; the amount of color can be measured and is proportional to the amount of antibody in the sample.

Wells containing samples negative for the target IgM antibodies remain colorless, indicating negative result.

Information | Packing | Specimen | Principle

HEV IgG ELISA Kit

General Information

Catalog No.:HEV 2924
Description:HEV IgG ELISA Kit
Category:Hepatitis Disease
Style:Microwell
Unit:Box
Specimen:serum, plasma
No. of Step:Two Step
Reading Time:30-30-10 Minutes
Cut Off:
Specification:96T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:Indirect ELISA

Packing Specification

Tests per Kit:96 Tests
Box Dimension:13.5 * 9.0 * 8.0 CM
Kits per CTN:40 Kits
Carton Dimension:59 * 45 * 38 CM
Tests per CTN (BULK):40 Tests
Carton Volume:0.100 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided

  • MICROWELL PLATE: 1 plate;
  • NEGATIVE CONTROL: 1 vial;
  • POSITIVE CONTROL SERUM: 1 vial;
  • SPECIMEN DILUENT: 1 vial;
  • HRP-CONJUGATE REAGENT: 1 vial;
  • WASH BUFFER: 1 bottle;
  • CHROMOGEN SOLUTION A: 1 vial;
  • CHROMOGEN SOLUTION B: 1 vial;
  • STOP SOLUTION: 1 vial;
  • PLASTIC SEALABLE BAG: 1 piece;
  • CARDBOARD PLATE COVER;
  • PACKAGE INSERTS: 1 copy.

Specimen

Plasma Collection

Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.

Separate the plasma by centrifugation.

Carefully withdraw the plasma into a new pre-labeled tube.

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum, plasma Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Indirect ELISA

ELISA Indirect Method Illustration

With indirect ELISA kit, polystyrene microwell strips are pre-coated with purified or recombinant viral antigens.

The antibody detection is achieved in two steps. During the first incubation step, the target antibodies, if present, will be bound to the solid phase pre-coated antigens.

The wells are washed to remove unbound serum proteins, and rabbit anti-human IgG antibodies (anti-IgG) conjugated to horseradish peroxidase (HRP) are added. During the second incubation step, these HRP-conjugated antibodies will be bound to any antigen-antibody complexes previously formed.

Then the unbound HRP-conjugate is then removed by washing. Chromogen solutions containing Tetramethyl benzidine (TMB) and urea peroxide are added to the wells. In presence of the antigen-antibody-anti-IgG (HRP) immuno complex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue colored product which turns blue color turns yellow after stopping the reaction with sulfuric acid, indicating a positive result.

Wells containing samples negative for the target antibodies remain colorless.

Information | Packing | Specimen | Principle

HEV Antigen ELISA Kit

General Information

Catalog No.:HEV 2934
Description:HEV Antigen ELISA Kit
Category:Hepatitis Disease
Style:Microwell
Unit:Box
Specimen:serum, plasma
No. of Step:Two Step
Reading Time:60-30-30 Minutes
Cut Off:
Specification:96T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:Double Antibody Sandwich ELISA

Packing Specification

Tests per Kit:96 Tests
Box Dimension:13.5 * 9.0 * 8.0 CM
Kits per CTN:40 Kits
Carton Dimension:59 * 45 * 38 CM
Tests per CTN (BULK):40 Tests
Carton Volume:0.100 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided

  • MICROWELL PLATE: 1 plate;
  • NEGATIVE CONTROL: 1 vial;
  • POSITIVE CONTROL SERUM: 1 vial;
  • SPECIMEN DILUENT: 1 vial;
  • HRP-CONJUGATE REAGENT: 1 vial;
  • WASH BUFFER: 1 bottle;
  • CHROMOGEN SOLUTION A: 1 vial;
  • CHROMOGEN SOLUTION B: 1 vial;
  • STOP SOLUTION: 1 vial;
  • PLASTIC SEALABLE BAG: 1 piece;
  • CARDBOARD PLATE COVER;
  • PACKAGE INSERTS: 1 copy.

Specimen

Plasma Collection

Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.

Separate the plasma by centrifugation.

Carefully withdraw the plasma into a new pre-labeled tube.

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum, plasma Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Double Antibody Sandwich ELISA

ELISA Double Antibody Sandwich Method Illustration

With this type of ELISA test kits, the detection target usually is hormone protein or viral antigen.

In the test kit, monoclonal or polyclonal antibodies specific to the target protein or antigen are used as capturer, which are pre-coated on the polystyrene microwell strips. The sample from the patient, such like urine, serum or plasma sample is added to the microwells. And a second antibody conjugated with horseradish peroxidase (HRP) is used as the detector, which then is added to the microwells. Usually, the samples and the second antibody conjugate are added to the microwell in the same time, thus it is a one-step assay process.

During incubation, the specific immuno-complex formed in case of presence of the target antigen or protein in the sample, and is captured on the solid phase.

After washing to remove sample serum proteins and unbound HRP-conjugate, Chromogen solutions containing tetramethyl benzidine (TMB) and urea peroxide are added to the wells. In presence of the antibody-antigen-antibody (HRP) "sandwich" immuno complex, the colorless Chromogens are hydrolyzed by the bounded HRP-conjugate to a blue colored product. The blue color turns yellow after stopping the reaction with sulfuric acid, indicating a positive result. The amount of color can be measured and is proportional to the amount of antigen or protein in the sample.

Wells containing samples negative for the target antigen or protein remain colorless.

Information | Packing | Specimen | Principle