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HIV ELISA Test Kits

We supply both HIV 1+2 antibody ELISA kit and HIV 1+2 Ag/Ab ELISA kit, which are intended to be used for massive blood screening, as an aid to detect antibodies and/or antigens of HIV-1 and/or HIV-2 infection.

anit-HIV ELISA Kit

HIV ELISA Kits

Atlas Link Technology Co., Ltd supplies both HIV 1+2 antibody ELISA kit, which can detect total antibody specific to both HIV type-1 and type-2, and the 4th generation HIV ELISA kit, which can detect the HIV antibodies and antigens simultaneously, with higher sensitivity and specificity.

These HIV ELISA tests for detection of HIV infection are characterized with high sensitivity, specificity and simple operation procedure. There are most appropriate for testing of large numbers of specimens and currently, internationally available are hundreds of HIV tests used in routine blood screening or clinical diagnosis.

HIV ELISA Kits, 3rd and 4th Generations

The third generation assays could detect all antibodies in sample (IgG, IgM, etc.) which significantly increases the assay’s sensitivity comparing to the previous generations. In addition, the detection of IgM antibodies that are present only during the early stages of infection, much shortens the antibody detection “window” period (the period of time in which there is no detectable antibody production), and compare to the second generation, “sandwich” tests could detect antibodies 11 days earlier.

To reduce even further the antibody detection “window” period, 4th generation HIV ELISAs that could simultaneously detect HIV antigens (p24) and antibodies have been developed and are commercially available since 1998. With detection of p24, the 4th generation tests shorten the “window” period to 16 days, or compare to the 3rd generation, HIV infection could be detected 8 days earlier.

HIV and AIDS

he human immunodeficiency virus (HIV) is a lentivirus that causes acquired immunodeficiency syndrome (AIDS). HIV attacks the immune system, resulting in a chronic, progressive illness and leading to life-threatening opportunistic infections.
There are two types of HIV: HIV-1 and HIV-2. HIV-1 has been isolated from patients with AIDS and AIDS related complex, and from healthy persons with high potential risk of developing AIDS. Patients with HIV-2 are found primarily in parts of West Africa.

Treatment with anti-retrovirals increases the life expectancy of people infected with HIV.

Add HIV 1/2 ELISA Kits to Inquiry Cart How to Use

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
HIV 3114 Anti-HIV ELISA Kit serum, plasma96 Boxed: 40 Kits
HIV 3124 HIV 1+2 Ag/Ab ELISA Kit serum, plasma96 Boxed: 40 Kits

Anti-HIV ELISA Kit

General Information

Catalog No.:HIV 3114
Description:Anti-HIV ELISA Kit
Category:Sexually Transmitted Disease
Style:Microwell
Unit:Box
Specimen:serum, plasma
No. of Step:Two Step
Reading Time:30-30-15 Minutes
Cut Off:
Specification:48T/96T/480T
Quan. or Qual.:Qualitative
Sensitivity:99.9%
Specificity:99.9%
Method:Double Antigen Sandwich ELISA

Packing Specification

Tests per Kit:96 Tests
Box Dimension:13.5 * 9.0 * 8.0 CM
Kits per CTN:40 Kits
Carton Dimension:59 * 45 * 38 CM
Tests per CTN (BULK):40 Tests
Carton Volume:0.100 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided

  • MICROWELL PLATE: 1 plate;
  • NEGATIVE CONTROL: 1 vial;
  • POSITIVE CONTROL SERUM: 1 vial;
  • SPECIMEN DILUENT: 1 vial;
  • HRP-CONJUGATE REAGENT: 1 vial;
  • WASH BUFFER: 1 bottle;
  • CHROMOGEN SOLUTION A: 1 vial;
  • CHROMOGEN SOLUTION B: 1 vial;
  • STOP SOLUTION: 1 vial;
  • PLASTIC SEALABLE BAG: 1 piece;
  • CARDBOARD PLATE COVER;
  • PACKAGE INSERTS: 1 copy.

Specimen

Plasma Collection

Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.

Separate the plasma by centrifugation.

Carefully withdraw the plasma into a new pre-labeled tube.

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum, plasma Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Double Antigen Sandwich ELISA

ELISA Double Antigen Sandwich Method Illustration

In double antigen sandwich ELISA kit, a recombinant or purified antigen is pre-coated on the polystyrene microwell strips, which acts as the capturer. And, a second antigen conjugated to tracer enzyme -horseradish peroxidase (HRP) is used as the detector. Depending on whether the specimen is added together with the HRP conjugate into the microwell or not, double antigen sandwich ELSIA are subdivided to one-step kit and two-step kit.

In one step kit, the sample is incubated in the microwells together with the second antigen HRP conjugate. In case of presence of the target antibody in the sample, the pre-coated and conjugated antigens will be bound to the two variable domains of the antibody during incubation, thus the specific antigen-antibody-antigen-HRP immuno complex will develop and be captured on the solid phase.

After washing to remove unbound sample and conjugates, Chromogen solutions containing tetramethyl benzidine (TMB) and urea peroxide are added to the wells and in presence of the "sandwich" immuno complex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue colored product, which turns yellow after stopping the reaction with sulfuric acid, indicating a positive result. The amount of color can be measured and is proportional to the amount of antibody in the sample.

Wells containing samples negative for target antibody remain colorless.

For two-step kit, the antibodies in the sample are first allowed to react with the pre-coated antigens during the first incubation. During this process, the target antibodies, if present, will be captured on the polystyrene microwell strips. In turn, they are detected by the addition of the HRP-conjugated antigens, with the same color development as described for one-step kit.

Information | Packing | Specimen | Principle

HIV 1+2 Ag/Ab ELISA Kit

General Information

Catalog No.:HIV 3124
Description:HIV 1+2 Ag/Ab ELISA Kit
Category:Sexually Transmitted Disease
Style:Microwell
Unit:Box
Specimen:serum, plasma
No. of Step:Two Step
Reading Time:60-30-15 Minutes
Cut Off:
Specification:96T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:D-antigen and D-antibody sandwich ELISA

Packing Specification

Tests per Kit:96 Tests
Box Dimension:13.5 * 9.0 * 8.0 CM
Kits per CTN:40 Kits
Carton Dimension:59 * 45 * 38 CM
Tests per CTN (BULK):40 Tests
Carton Volume:0.100 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided

  • MICROWELL PLATE: 1 plate;
  • NEGATIVE CONTROL: 1 vial;
  • POSITIVE CONTROL SERUM: 1 vial;
  • SPECIMEN DILUENT: 1 vial;
  • HRP-CONJUGATE REAGENT: 1 vial;
  • WASH BUFFER: 1 bottle;
  • CHROMOGEN SOLUTION A: 1 vial;
  • CHROMOGEN SOLUTION B: 1 vial;
  • STOP SOLUTION: 1 vial;
  • PLASTIC SEALABLE BAG: 1 piece;
  • CARDBOARD PLATE COVER;
  • PACKAGE INSERTS: 1 copy.

Specimen

Plasma Collection

Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.

Separate the plasma by centrifugation.

Carefully withdraw the plasma into a new pre-labeled tube.

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum, plasma Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of D-antigen and D-antibody sandwich ELISA

A simple combination of double antigen sandwich and double antibody sandwich in a single test kit.

Information | Packing | Specimen | Principle