NOVAtest brand

Menu Search

TB IgG and TB-IGRA ELISA Kits

TB Correlative IFN-γ Release Assay (TB-IGRA) is a newly developed quantitative test kits; TB IgG ELISA kits is used to detect TB IgG antibody, both are used in the diagnosis of tuberculosis infection.

TB-IGRA ELISA Kit

TB Correlative IFN-γ Release Assay (TB-IGRA)

Tuberculosis (TB) Rapid Test Kit

TB-IGRA ELISA kit is an enzyme-linked immunosorbent assay kit for the quantitative detection of Interferon Gamma (IFN-y) that respond to in-vitro stimulation by mycobacterium tuberculosis antigens in human whole blood, with detection concentration range of 2~400pg/ml. It is intended to be used as an efficient aid in the diagnosis of tuberculosis (TB) infections, including both latent tuberculosis infection (LTBI) and tuberculosis (TB) disease.

Merits of TB-IGRA ELISA kit

IGRA measures a person's immune reactivity to M.tuberculosis. When infected with mycobacterium tuberculosis, specific T-lymphocytes will appear in the blood of the patient, which will be stimulated to proliferate and release cytokines such as IFN-γ, when they are mixed with antigens derived from M.Tuberculosis. The concentration of IFN-γ is positively related with the quantity of tuberculosis antigens.

The antigens selected for stimulation are specific fragments that pathogenic Mycobacterium tuberculosis virus contains, while BCG vaccine and other Mycobacterium don't contain. This greatly improves the specificity of the TB-IGRA Assay kit. And this TB Assay kit is easier to operate than other methods like PCR, bacterial culture and SPOT.

This TB-IGRA ELISA kit can be used in the following realms:

  • Active tuberculosis detection;
  • Latent tuberculosis detection;
  • TB therapeutic effect monitor;
  • Medication instruction;
  • Prevent TB cross infection.

Tuberculosis and TB Test Kits

Tuberculosis is a highly infectious disease caused by various strains of mycobacteria, usually Mycobacterium tuberculosis (MTB or TB). It is a small bacillus that can withstand weak disinfectants and can survive in a dry state for weeks. TB is transmitted almost exclusively by aerosol from the respiratory secretions of patients with active M. tuberculosis.

As present, Tuberculosis remains an important socio-economical and medical problem throughout the world. The early detection of tuberculosis disease will help to reduce and chance of further spread, and will help to the cure of the infected. In this way, the invitro-diagnostic (IVD) TB testing devices are contributing to the control of tuberculosis spreading.

Add Tuberculosis ELISA Kits to Inquiry Cart How to Use

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
TB 8124 TB IgG ELISA Kit serum, whole blood96 Boxed: 40 Kits
TB 8134 TB Correlative IFN-γ Release Assay serum, whole blood96 Boxed: 40 Kits

TB IgG ELISA Kit

General Information

Catalog No.:TB 8124
Description:TB IgG ELISA Kit
Category:Respiratory Tract Disease
Style:Microwell
Unit:Box
Specimen:serum, whole blood
No. of Step:Two Step
Reading Time:60-15 Minutes
Cut Off:
Specification:96T
Quan. or Qual.:Qualitative
Sensitivity:89%
Specificity:93%
Method:Indirect ELISA

Packing Specification

Tests per Kit:96 Tests
Box Dimension:13.5 * 9.0 * 8.0 CM
Kits per CTN:40 Kits
Carton Dimension:59 * 45 * 38 CM
Tests per CTN (BULK):40 Tests
Carton Volume:0.100 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided

  • MICROWELL PLATE: 1 plate;
  • NEGATIVE CONTROL: 1 vial;
  • POSITIVE CONTROL SERUM: 1 vial;
  • SPECIMEN DILUENT: 1 vial;
  • HRP-CONJUGATE REAGENT: 1 vial;
  • WASH BUFFER: 1 bottle;
  • CHROMOGEN SOLUTION A: 1 vial;
  • CHROMOGEN SOLUTION B: 1 vial;
  • STOP SOLUTION: 1 vial;
  • PLASTIC SEALABLE BAG: 1 piece;
  • CARDBOARD PLATE COVER;
  • PACKAGE INSERTS: 1 copy.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

Whole Blood Collection

Whole blood can be collected with blood collection tube with venipuncture into blood collection tube (containing EDTA, citrate or heparin).

Or, by lancet lancing. Clean the area to be lanced with an alcohol swab. Squeeze the end of the fingertip and pierce with a sterile lancet. Wipe away the first drop of blood with sterile gauze or cotton. Take a dropper provided, while gently squeezing the dropper tube, immerse the open end in the blood drop and then gently release the pressure to draw blood into the dropper.

serum, whole blood Storage Condition

Test specimens as soon as possible after collecting.

The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times.

Prior to testing, bring frozen specimens to room temperature slowly and mix gently.

Specimens containing visible particulate matter should be clarified by centrifugation before testing.

Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Indirect ELISA

ELISA Indirect Method Illustration

With indirect ELISA kit, polystyrene microwell strips are pre-coated with purified or recombinant viral antigens.

The antibody detection is achieved in two steps. During the first incubation step, the target antibodies, if present, will be bound to the solid phase pre-coated antigens.

The wells are washed to remove unbound serum proteins, and rabbit anti-human IgG antibodies (anti-IgG) conjugated to horseradish peroxidase (HRP) are added. During the second incubation step, these HRP-conjugated antibodies will be bound to any antigen-antibody complexes previously formed.

Then the unbound HRP-conjugate is then removed by washing. Chromogen solutions containing Tetramethyl benzidine (TMB) and urea peroxide are added to the wells. In presence of the antigen-antibody-anti-IgG (HRP) immuno complex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue colored product which turns blue color turns yellow after stopping the reaction with sulfuric acid, indicating a positive result.

Wells containing samples negative for the target antibodies remain colorless.

Information | Packing | Specimen | Principle

TB Correlative IFN-γ Release Assay

General Information

Catalog No.:TB 8134
Description:TB Correlative IFN-γ Release Assay
Category:Respiratory Tract Disease
Style:Microwell
Unit:Box
Specimen:serum, whole blood
No. of Step:One Step
Reading Time:10 min-16 Hour
Cut Off:
Specification:---
Quan. or Qual.:Quantitative
Sensitivity:89%
Specificity:93%
Method:Double Antibody Sandwich ELISA

Packing Specification

Tests per Kit:96 Tests
Box Dimension:13.5 * 9.0 * 8.0 CM
Kits per CTN:40 Kits
Carton Dimension:59 * 45 * 38 CM
Tests per CTN (BULK):40 Tests
Carton Volume:0.100 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided

  • MICROWELL PLATE: 1 plate;
  • NEGATIVE CONTROL: 1 vial;
  • POSITIVE CONTROL SERUM: 1 vial;
  • SPECIMEN DILUENT: 1 vial;
  • HRP-CONJUGATE REAGENT: 1 vial;
  • WASH BUFFER: 1 bottle;
  • CHROMOGEN SOLUTION A: 1 vial;
  • CHROMOGEN SOLUTION B: 1 vial;
  • STOP SOLUTION: 1 vial;
  • PLASTIC SEALABLE BAG: 1 piece;
  • CARDBOARD PLATE COVER;
  • PACKAGE INSERTS: 1 copy.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

Whole Blood Collection

Whole blood can be collected with blood collection tube with venipuncture into blood collection tube (containing EDTA, citrate or heparin).

Or, by lancet lancing. Clean the area to be lanced with an alcohol swab. Squeeze the end of the fingertip and pierce with a sterile lancet. Wipe away the first drop of blood with sterile gauze or cotton. Take a dropper provided, while gently squeezing the dropper tube, immerse the open end in the blood drop and then gently release the pressure to draw blood into the dropper.

serum, whole blood Storage Condition

Test specimens as soon as possible after collecting.

The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times.

Prior to testing, bring frozen specimens to room temperature slowly and mix gently.

Specimens containing visible particulate matter should be clarified by centrifugation before testing.

Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Double Antibody Sandwich ELISA

ELISA Double Antibody Sandwich Method Illustration

With this type of ELISA test kits, the detection target usually is hormone protein or viral antigen.

In the test kit, monoclonal or polyclonal antibodies specific to the target protein or antigen are used as capturer, which are pre-coated on the polystyrene microwell strips. The sample from the patient, such like urine, serum or plasma sample is added to the microwells. And a second antibody conjugated with horseradish peroxidase (HRP) is used as the detector, which then is added to the microwells. Usually, the samples and the second antibody conjugate are added to the microwell in the same time, thus it is a one-step assay process.

During incubation, the specific immuno-complex formed in case of presence of the target antigen or protein in the sample, and is captured on the solid phase.

After washing to remove sample serum proteins and unbound HRP-conjugate, Chromogen solutions containing tetramethyl benzidine (TMB) and urea peroxide are added to the wells. In presence of the antibody-antigen-antibody (HRP) "sandwich" immuno complex, the colorless Chromogens are hydrolyzed by the bounded HRP-conjugate to a blue colored product. The blue color turns yellow after stopping the reaction with sulfuric acid, indicating a positive result. The amount of color can be measured and is proportional to the amount of antigen or protein in the sample.

Wells containing samples negative for the target antigen or protein remain colorless.

Information | Packing | Specimen | Principle