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anti-TP ELISA Kit

Anti-TP ELISA kit is an enzyme-linked immunosorbent assay, intended for screening of blood donors, and as an aid for the diagnosis and management of clinical conditions known as syphilis.

anti-TP ELISA Kit

Anti-TP ELISA Kit

This anti-TP ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for qualitative determination of the antibodies to Treponema pallidum (TP) in human serum or plasma. It is intended for screening of blood donors and as an aid for the diagnosis and management of clinical conditions known as syphilis.

Assay Principle of Anti-TP ELISA

With this Syphilis ELISA kit, the detection of anti-TP antibodies is achieved by antigen ”sandwich” enzyme-linked method, where ┬ápolystyrene microwell strips are pre-coated with recombinant Treponema pallidum antigens expressed in E. coli. The sample is incubated in the microwells together with recombinant TP antigens conjugated to horseradish peroxidase (HRP-Conjugate). The pre-coated antigens express the same epitopes as the HRP-Conjugate antigens, but are expressed in different hosts. In case of presence of anti-TP in the sample, during incubation the pre-coated and conjugated antigens will be bound to the two variable domains of the antibody and the ┬áspecific antigens-antibody immunocomplex is captured on the solid phase.

After washing to remove sample and unbound conjugates, Chromogen solutions containing tetramethylbenzidine (TMB) and urea peroxide are added into the wells. In presence of the antigen-antibody-antigen “sandwich” complex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid.

The amount of color can be measured and is proportional to the amount of antibody in the sample. Wells containing samples negative for anti-TP remain colorless.

Syphilis and TP Virus

Syphilis is a disease caused by Spirochete bacterium called Treponema pallidum (TP). If untreated, the organisms move throughout the body and can cause damage to many organs, making syphilis a life-threatening disease if not treated early enough.

The first detectable response to infection is the production of specific antitreponemal IgM, which can be detected within 4 to 7 days after the chancre appears and until the end of the second week of infection; antitreponemal IgG appears at about four weeks later. By the time that symptoms develop, most patients have detectable IgG and IgM.

Add Treponema Pallidum (Syphilis) ELISA Kits to Inquiry Cart How to Use

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
TP 3314 Anti-Syphilis ELISA Kit serum, plasma96 Boxed: 40 Kits

Anti-Syphilis ELISA Kit

General Information

Catalog No.:TP 3314
Description:Anti-Syphilis ELISA Kit
Category:Sexually Transmitted Disease
Style:Microwell
Unit:Box
Specimen:serum, plasma
No. of Step:One Step
Reading Time:60-15 Minutes
Cut Off:
Specification:96T/480T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:Double Antigen Sandwich ELISA

Packing Specification

Tests per Kit:96 Tests
Box Dimension:13.5 * 9.0 * 8.0 CM
Kits per CTN:40 Kits
Carton Dimension:59 * 45 * 38 CM
Tests per CTN (BULK):40 Tests
Carton Volume:0.100 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided

  • MICROWELL PLATE: 1 plate;
  • NEGATIVE CONTROL: 1 vial;
  • POSITIVE CONTROL SERUM: 1 vial;
  • SPECIMEN DILUENT: 1 vial;
  • HRP-CONJUGATE REAGENT: 1 vial;
  • WASH BUFFER: 1 bottle;
  • CHROMOGEN SOLUTION A: 1 vial;
  • CHROMOGEN SOLUTION B: 1 vial;
  • STOP SOLUTION: 1 vial;
  • PLASTIC SEALABLE BAG: 1 piece;
  • CARDBOARD PLATE COVER;
  • PACKAGE INSERTS: 1 copy.

Specimen

Plasma Collection

Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.

Separate the plasma by centrifugation.

Carefully withdraw the plasma into a new pre-labeled tube.

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum, plasma Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Double Antigen Sandwich ELISA

ELISA Double Antigen Sandwich Method Illustration

In double antigen sandwich ELISA kit, a recombinant or purified antigen is pre-coated on the polystyrene microwell strips, which acts as the capturer. And, a second antigen conjugated to tracer enzyme -horseradish peroxidase (HRP) is used as the detector. Depending on whether the specimen is added together with the HRP conjugate into the microwell or not, double antigen sandwich ELSIA are subdivided to one-step kit and two-step kit.

In one step kit, the sample is incubated in the microwells together with the second antigen HRP conjugate. In case of presence of the target antibody in the sample, the pre-coated and conjugated antigens will be bound to the two variable domains of the antibody during incubation, thus the specific antigen-antibody-antigen-HRP immuno complex will develop and be captured on the solid phase.

After washing to remove unbound sample and conjugates, Chromogen solutions containing tetramethyl benzidine (TMB) and urea peroxide are added to the wells and in presence of the "sandwich" immuno complex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue colored product, which turns yellow after stopping the reaction with sulfuric acid, indicating a positive result. The amount of color can be measured and is proportional to the amount of antibody in the sample.

Wells containing samples negative for target antibody remain colorless.

For two-step kit, the antibodies in the sample are first allowed to react with the pre-coated antigens during the first incubation. During this process, the target antibodies, if present, will be captured on the polystyrene microwell strips. In turn, they are detected by the addition of the HRP-conjugated antigens, with the same color development as described for one-step kit.

Information | Packing | Specimen | Principle