EDDP Urine Test Kits
EDDP Urine Test kit is a one-step rapid test for the qualitative detection of methadone metabolite, ethylenediamine-dimethyl phosphinic acid, in human urine specimen at cut-off of 100 ng/mL.

EDDP Urine Screening Kits
The EDDP Rapid Test kit (Urine) is a rapid visual immunoassay for the qualitative, presumptive detection of Methadone metabolite, i.e. ethylenediamine-dimethyl phosphinic acid, in human urine specimens at the cut-off concentration of 100 100 ng/mL. The EDDP urine test is used for screening purpose only; for positive results, more advanced testing method should be used before final conclusion is reached.
Reagents Used in EDDP Test Strip
Each EDDP test device consists of a reagent strip. The amount of each antigen and/or antibody coated on the EDDP test strip is less than 0.001 mg for antigen conjugates and goat anti-rabbit IgG antibodies, and less than 0.0015 mg for antibody components.
The control zone of each EDDP test contains goat anti-rabbit IgG antibody. The test zone of each test contains drug-bovine protein antigen conjugate, and the conjugate pad of each test contains monoclonal anti-drug antibody and rabbit antibody-colored particle complex.
Precautions on EDDP Test Kit
- For professional in vitro diagnostic use only.
- Do not use after the expiration date indicated on the package. Do not use the test if the foil pouch is damaged. Do not reuse tests.
- This EDDP test kit contains products of animal origin. Certified knowledge of the origin and/or sanitary state of the animals does not completely guarantee the absence of transmissible pathogenic agents. It is therefore recommended that these products be treated as potentially infectious, and handled observing usual safety precautions (e.g., do not ingest or inhale).
- Avoid cross-contamination of specimens by using a new specimen collection container for each specimen obtained.
- Read the entire procedure carefully prior to testing.
- Do not eat, drink or smoke in the area where the specimens and EDDP urine screening kits are handled. Handle all specimens as if they contain infectious agents. Observe established precautions against microbiological hazards throughout the procedure and follow standard procedures for proper disposal of specimens. Wear protective clothing such as laboratory coats, disposable gloves and eye protection when specimens are assayed.
- Do not interchange or mix reagents from different lots.
- Humidity and temperature can adversely affect results.
Why Testing EDDP
EDDP is a synthetic analgesic drug that is originally used in the treatment of narcotic addicts. Among the psychological effects induced by using methadone are analgesia, sedation and respiratory depression. Overdose of methadone may cause coma or even death. It is administered orally or intravenously and is metabolized in the liver. The kidneys are a major route of methadone excretion.
Methadone has a biological half-life of 16-50 hours. EDDP (2-Ethyliden-1,5-Dimethyl-3,3-Diphenylpyrrolidine) is the most important metabolite of methadone. The part of the unchanged excreted methadone is variable and depends on the urine’s pH value, dose, and the patient’s metabolism. Therefore, the detection of the metabolite EDDP instead of methadone itself is useful in determination of methadone usage, because interferences of the patient’s metabolism are avoided.
Cut-off of Drug Test Kits
No. | Abbr. | Full Name | Cut-off (ng/ml) |
---|---|---|---|
1 | AMP | drug Amphetamine | 1000 |
2 | BAR | drug SecoBarbital | 300 |
3 | BUP | drug Buprenorphine | 10 |
4 | BZO | Oxazepam Benzodiazepines | 300 |
5 | COC | drug Cocaine | 300 |
6 | COT | drug Cotinine | 200 |
7 | EDDP | ethylenediamine-dimethyl phosphinic acid | 100 |
8 | FYL | drug Fentanyl | 200 |
9 | K2 | Synthetic Cannabis | 50 |
10 | KET | drug Ketamine | 1000 |
11 | LSD | Lysergic acid diethylamide | 50 |
12 | MDMA | 3,4-methylenedioxy methamphetamine | 500 |
13 | MET | drug Methamphetamine | 1000 |
14 | MOR | drug Morphine | 300 |
15 | MQL | drug Methaqualone | 300 |
16 | MTD | drug Methadone | 300 |
17 | OPI | Opiates Morphine | 2000 |
18 | OXY | drug Oxycodone | 100 |
19 | PCP | drug Phencyclidine | 25 |
20 | PPX | drug Propoxyphene | 300 |
21 | TCA | Tricyclic Antidepressants | 1000 |
22 | THC | Marijuana | 50 |
23 | TML | drug Tramadol | 100 |
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