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cTnI Rapid Test Kits

Troponin-I rapid test cassette is one most often used rapid test device in the emergent diagnosis of the heart attack.

cTnI Rapid Test Cassette

Intended Use

One-step Cardiac Troponin-I (cTnI) Rapid test cassette is an in-vitro diagnostic (IVD) test kit, based on the immune-chromatographic assay principle, which is intended to be used in the diagnosis of Acute Myocardial Infarction. cTnI rapid test kit is one most common test device used in the diagnosis of the heart attack.

Merits of cTnI Rapid Test

  • Rapid Troponin-I tests are highly sensitive and specific;
  • Troponin-I test cassettes are easy to perform;
  • Qualitative results of cTnI cassettes are read visually without any instrument;
  • No other reagent is needed for these cardiac cTnI rapid test kits;
  • With troponin-I test cassettes, it takes less than 15 minutes to be sure.

These merits make it suitable to be used in the POCT settings such like in an ambulance or an emergency room, and is effective at both the cost and the life-saving efficiency.

What cTnI is?

Cardiac Troponin I, short as cTnI, is a protein which uniquely found in heart muscle. When heart attack happens, this protein will be released into the blood. This Troponin-I remains at elevated level for 6-10 days, thus providing for a longer window of detection for cardiac injury. This makes the cTnI test kit a most common heart attack test kit.

AMI Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
cTnI 721 Troponin-I Rapid Test Card serum/WB/plasma 25 Bulk: 1600 PCS
Boxed: 40 Boxes
How to Use

Troponin-I Rapid Test Card

General Information

(IVD Kit »-» Health Monitoring -» One Step Lateral Flow Immunochromatographic Assay)

Catalog No.:cTnI 721
Description:Troponin-I Rapid Test Card
Category:Health Monitoring
Style:Card
Unit:PCS
Specimen:serum/WB/plasma
No. of Step:One Step
Reading Time:10 Minutes
Cut Off:80 ng/ml
Specification:4.0 mm
Quan. or Qual.:Qualitative
Sensitivity:98.5%
Specificity:98.4%
Method:Lateral Flow Double Antibody Sandwich

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:40 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1600 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):14.0 KG
GW per CTN (Bulk):16.5 KG

Materials Provided in the Test Kit

  • Test cassette in single foil pouch with desiccant;
  • Disposable sample dropper;
  • Instruction leaflet.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

Whole Blood Collection

Whole blood can be collected with blood collection tube with venipuncture into blood collection tube (containing EDTA, citrate or heparin).

Or, by lancet lancing. Clean the area to be lanced with an alcohol swab. Squeeze the end of the fingertip and pierce with a sterile lancet. Wipe away the first drop of blood with sterile gauze or cotton. Take a dropper provided, while gently squeezing the dropper tube, immerse the open end in the blood drop and then gently release the pressure to draw blood into the dropper.

Plasma Collection

Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.

Separate the plasma by centrifugation.

Carefully withdraw the plasma into a new pre-labeled tube.

serum/WB/plasma Storage Condition

Test specimens as soon as possible after collecting.

The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times.

Prior to testing, bring frozen specimens to room temperature slowly and mix gently.

Specimens containing visible particulate matter should be clarified by centrifugation before testing.

Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Lateral Flow Double Antibody Sandwich

GICA Double Antibody Sandwich Method Illustration

In the double antibody sandwich lateral flow chromatographic rapid test, two antibodies are used to detect viral protein or antigen.

In the test band region, antibody specific to the target antigen or protein is immobilized. In the end of the membrane strip where the samples are added, another antibody conjugated with colloidal gold particles is embedded.

During the testing process, specimen from the patient is added to the end of the membrane strip. The antibody conjugate particles will resolve into the specimen, where the antibody conjugate will react and bound to the target protein or viral antigen, if present.

This antigen-antibody conjugate complex then moves along the strip to the testing band region, under capillary effect. The immobilized antibody in the test line region then bound to another binding site on the antigen complex, and the antibody-antigen-antibody complex is fixed on test band region.

When the complex congregates, the highly concentrated colloidal gold particles will develop some red or pink color, indicate a positive result.

If there is no target protein or antigen in the specimen, the colloidal gold particles will not congregate and no color will develop, indicating a negative result.

Information | Packing | Specimen | Principle