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MiA Urine Test

This Micro Albumin urine rapid test kit is used as a prognostic marker of kidney damage in its early stage.

Micro Albumin test strip and cassette

Intended Use

Micro Albumin urine test kit is a chromatographic immunoassay for the semi-quantitative detection of micro-albumin in human urine specimen based on immuno competitive inhibition principle. The test result can be used in many clinical diagnostic situations, especially as a prognostic marker of kidney damages in its early stage.

Testing Principle of MiA Rapid Test

After added, the urine specimen will dissolve the pre-coated micro-albumin-specific monoclonal antibody labeled with colloidal gold. The micro-albumin, if present, will react with the conjugated antibody. If there is enough micro albumins in the urine, all the colloidal gold conjugated antibody will be bound, leaving no or very small amount of colloidal gold conjugated antibody to react with the micro albumin pre-coated in the test (T) region. So, no colloidal gold particle will be bound in the test region, thus no color develops in the test region, indicating a strong positive result (>50mg/L).

If the albumin concentration in the specimen is not high enough, and there are some colloidal gold conjugated antibody left to be bound in the test region, a dim pink line will develop in the test region, indicating the concentration is higher than normal value of 20mg/L, but lower than 50 mg/L. And, if test line is as dark as, or darker than, the reference line, it means the concentration is normal (<20 mg/L).

Micro-Albumin as Health Indicator

When the permeability for albumin in the renal glomerulus is abnormally high, micro-albumin in the blood will leak into the urine. Usually, the level of albumin produced by microalbuminuria is lower than that can be detected by our urine reagent strips. Usually, when the level is above 100 mg/L, it is considered serious microalbuminuria; when it is over 300 mg/L, it is then called macroalbuminuria or albuminuria.

The level of micro albumin can be used in many situations in clinical diagnosis realm, among them including: as an indicator of subclinical cardiovascular disease; as a marker of vascular endothelial dysfunction; as an important prognostic marker for kidney disease, such as diabetes mellitus and hypertension; and, as a risk factor for venous thromboembolism.

Micro Albumin Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
MiA 151 Micro-Albumin Urine Strip urine 100 Bulk: 6000 PCS
Boxed: 48 Boxes
MiA 152 Micro-Albumin Urine Card urine 25 Bulk: 1600 PCS
Boxed: 40 Boxes
How to Use

Micro-Albumin Urine Strip

General Information

(IVD Kit »-» Health Monitoring -» One Step Lateral Flow Immunochromatographic Assay)

Catalog No.:MiA 151
Description:Micro-Albumin Urine Strip
Category:Health Monitoring
Style:Dipstick
Unit:PCS
Specimen:urine
No. of Step:One Step
Reading Time:5-10 Minutes
Cut Off:20mg/L, 50 mg/L
Specification:3.0 mm
Quan. or Qual.:Semi-Quantitative
Sensitivity:
Specificity:
Method:Lateral Flow Competitive Binding

Packing Specification

Tests per Kit:100 Tests
Box Dimension:21.0 * 12.5 * 5.5 CM
Kits per CTN:48 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):6000 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):16.0 KG

Materials Provided in the Test Kit

  • Lateral Flow immunochromatographic assay (GICA) Strip;
  • Instruction leaflet;
  • Disposable urine cup (optional).

Specimen

Urine Collection

A urine specimen must be collected in a clean and dry container.

For hormone test such as HCG prenancy test, the first urine specimen in the morning is preferred since it generally contains the highest concentration of hormone, although urine specimens collected at any time of the day may be used.

Urine specimens exhibiting visible particles should be centrifuged, filtered, or allowed to settle to obtain clear specimen for testing.

urine Storage Condition

Urine specimens may be stored at 2-8°C for up to 48 hours prior to testing. For long-term storage, specimens may be frozen and stored below -20°C.

Frozen specimens should be thawed and mixed before testing.

Testing Principle of Lateral Flow Competitive Binding

GICA Competitive Method Illustration

Drug abuse test kits are based on the Lateral Flow Competitive Binding rapid test principle.

In the test kit, drug-protein conjugates (purified bovine albumin) are immobilized in the test line region. And the drug specific antibody conjugated with colloidal gold particles is put in the membrane of the sample adding region.

During testing, when the urine specimen is added to the sample adding region, the antibody conjugate particles will come into the urine specimen, where the antibody conjugate will react with the drug. If there is no target drug in the urine specimen, or the concentration is lower than the cut-off, the drug can not bind all the binding sites of the drug specific antibody colloidal gold conjugate.

The unbonded colloidal gold conjugate will be captured by the immobilized drug in the testing line region. When the antibody conjugated colloidal gold particle congregate, a red or pink color will develop, indicating a negative result.

If the concentration of the drug is higher than the cut off value of the test kit, the drug will saturate all the binding sites of the antibody conjugate. Then, when the mixed fluid moves to the test region under capillary effect, there will be no free antibody particles to bind with the immobilized drug, and no color will develop, indicating a positive result.

Information | Packing | Specimen | Principle

Micro-Albumin Urine Card

General Information

(IVD Kit »-» Health Monitoring -» One Step Lateral Flow Immunochromatographic Assay)

Catalog No.:MiA 152
Description:Micro-Albumin Urine Card
Category:Health Monitoring
Style:Card
Unit:PCS
Specimen:urine
No. of Step:One Step
Reading Time:5-10 Minutes
Cut Off:20mg/L, 50 mg/L
Specification:4.0 mm
Quan. or Qual.:Semi-Quantitative
Sensitivity:
Specificity:
Method:Lateral Flow Competitive Binding

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:40 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1600 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):14.0 KG
GW per CTN (Bulk):16.5 KG

Materials Provided in the Test Kit

  • Test cassette in single foil pouch with desiccant;
  • Disposable sample dropper;
  • Instruction leaflet.

Specimen

Urine Collection

A urine specimen must be collected in a clean and dry container.

For hormone test such as HCG prenancy test, the first urine specimen in the morning is preferred since it generally contains the highest concentration of hormone, although urine specimens collected at any time of the day may be used.

Urine specimens exhibiting visible particles should be centrifuged, filtered, or allowed to settle to obtain clear specimen for testing.

urine Storage Condition

Urine specimens may be stored at 2-8°C for up to 48 hours prior to testing. For long-term storage, specimens may be frozen and stored below -20°C.

Frozen specimens should be thawed and mixed before testing.

Testing Principle of Lateral Flow Competitive Binding

GICA Competitive Method Illustration

Drug abuse test kits are based on the Lateral Flow Competitive Binding rapid test principle.

In the test kit, drug-protein conjugates (purified bovine albumin) are immobilized in the test line region. And the drug specific antibody conjugated with colloidal gold particles is put in the membrane of the sample adding region.

During testing, when the urine specimen is added to the sample adding region, the antibody conjugate particles will come into the urine specimen, where the antibody conjugate will react with the drug. If there is no target drug in the urine specimen, or the concentration is lower than the cut-off, the drug can not bind all the binding sites of the drug specific antibody colloidal gold conjugate.

The unbonded colloidal gold conjugate will be captured by the immobilized drug in the testing line region. When the antibody conjugated colloidal gold particle congregate, a red or pink color will develop, indicating a negative result.

If the concentration of the drug is higher than the cut off value of the test kit, the drug will saturate all the binding sites of the antibody conjugate. Then, when the mixed fluid moves to the test region under capillary effect, there will be no free antibody particles to bind with the immobilized drug, and no color will develop, indicating a positive result.

Information | Packing | Specimen | Principle