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HPV IgG Test Kit

HPV IgG Dot Assay rapid test kit are intended to be used to detect HPV infection, as a measure to reduce the chance of related anogenital cancers.

HPV IgG Dot Assay Kit

HPV Dot Assay Kit

HPV IgG Dot Assay kit is immunoassay rapid test kit, based on the principle of Dot Immuno Gold Filtration Assay (DIGFA), which adopted purified monoclonal antigens to detect IgG antibody specific to Human Papilloma Virus (HPV) in human serum and plasma, and are intended to be used as an aid in the diagnosis of HPV infection.

Why HPV Testing Matter

Most HPV infections in young females are temporary, and cause little chronic lesions. About 90% of infections disappear within 2 years. But in the remaining less than 10%, the risk of developing precancerous lesions of the cervix is high, which can progress to invasive cervical cancer. This process usually takes 15-20 years, providing many opportunities for detection and treatment of the pre-cancerous lesion. Progression to invasive cancer can be effectively prevented after standard prevention strategies are applied. So, regular testing of HPV infection is a good method to avoid the anogenital cancers such as cervical cancer.

HPV and Cancer

The human papillomavirus (HPV) is a member of the papillomavirus family. The majority of the over 100 known types of HPV causes no symptoms and is referred to as low risk HPV; while high risk HPVs can cause warts, and even can lead to invasive cancers on cervix, vulva, vagina and anus in women, or cancers of the anus and penis in men. HPV can be transmitted by skin to skin contact, sexual action or even kissing. More than 30 types of HPV are typically transmitted through sexual contact and infect the anogenital region. Among them, at least 13 types can lead to cervical cancer or other anogenital cancers.

Human Papillomavirus Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
HPV 3512 HPV IgM Dot Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
HPV 3522 HPV IgG Dot Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
How to Use

HPV IgM Dot Assay

General Information

(IVD Kit »-» Sexually Transmitted Disease -» Dot Immuno Gold Filtration Assay)

Catalog No.:HPV 3512
Description:HPV IgM Dot Assay
Category:Sexually Transmitted Disease
Style:DOT Card
Unit:PCS
Specimen:serum
No. of Step:Two Step
Reading Time:3 Minutes
Cut Off:
Specification:25T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:DGFIA Capture

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:36 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1200 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • Filtration assay device;
  • Washing buffer Gold colloidal Conjugate solution;
  • Disposable dropper for sample transfer;
  • Instruction for use.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of DGFIA Capture

DGFIA Capture Method Illustration

For the detection of IgM-class antibodies, capture technique has its advantage in that it can reduce the interference from the IgG class, thus increase the specificity of the test kit.

In a capture dot filtration test kit, antibodies directed to human IgM antibody (anti-i chain) are pre-coated in the test region on the millipore filtering membrane. When specimen is added, the IgM, if present, will be captured by this secondary antibody, anti-IgM.

After washing away the specimen, especially the IgG antibody, the antigen conjugated with colloidal gold particle will be added. If the IgM antibody was captured in the prior step, the IgM will bind to the antigen conjugate. When the colloidal gold particles congregate, a red or pink color will develop, indicating a positive result.

If no IgM in the specimen, this complex will not develop, the antigen conjugated colloidal gold particles will not congregate, and no color will develop in the test region, indicating a negative result.

Information | Packing | Specimen | Principle

HPV IgG Dot Assay

General Information

(IVD Kit »-» Sexually Transmitted Disease -» Dot Immuno Gold Filtration Assay)

Catalog No.:HPV 3522
Description:HPV IgG Dot Assay
Category:Sexually Transmitted Disease
Style:DOT Card
Unit:PCS
Specimen:serum
No. of Step:Two Step
Reading Time:3 Minutes
Cut Off:
Specification:25T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:DGFIA indirect

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:36 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1200 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • Filtration assay device;
  • Washing buffer Gold colloidal Conjugate solution;
  • Disposable dropper for sample transfer;
  • Instruction for use.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of DGFIA indirect

DGFIA Indirect Method Illustration

With indirect Dot filtration test kit, the millipore filtering membrane is pre-coated with purified or recombinant viral antigens. The detecting conjugate reagent is stored in a vial, whose main component is rabbit anti human IgG (anti-IgG) antibodies, which is conjugated with colloidal gold particles.

During the first step, sample is added to the sample well in the test cassette. The target IgG antibodies, if present, will be captured by the pre-coated antigens.

The wells are washed to remove unbound serum proteins. Then, the colloidal gold conjugate is added. If the target IgG antibodies have been captured in the first step, the IgG antibody will react with the added secondary antibody, anti-IgG conjugate, and red color will develop, indicating a positive result.

Information | Packing | Specimen | Principle