Leishmania IgG/IgM Rapid Test Cassette

Leishmania IgG/IgM Rapid Test Cassette

Leishmania IgG/IgM Rapid Test Kit is a lateral flow immunoassay for the simultaneous detection and differentiation of the IgG and IgM antiboidies to the Leishmania donovani (L.donovani) in human serum or plasma. This Leishmania blood test is intended to be used as a blood screening test and as an aid in the diagnosis of the disease of Visceral leishmaniasis, also known as Kala-Azar. Any reactive specimen with this rapid Kala-Azar Test kit must be confirmed with alternative testing method(s) together with other clinical diagnosis.

Principle

The Leishmania IgG/IgM Rapid Test cassette consists of: 1) a burgundy colored conjugate pad containing recombinant L.donovani antigen conjugated with colloid gold (Leishmania conjugates) and rabbit IgG-gold conjugates, 2) a nitrocellulose membrane strip containing two test bands (1 and 2 bands) and a control band (C band). The band 1 is pre-coated with monoclonal anti-human IgM for the detection of anti-L.donovani IgM, band 2 is pre-coated with reagent for the detection of anti-L.donovani IgG, and the C band is pre-coated with goat anti rabbit IgG..

When an adequate volume of test specimen is dispensed into the sample well of the cassette, the specimen migrates by capillary action across the cassette. The L.donovani IgM, if present in the specimen, will bind to the Leishmania conjugates. The immunocomplex is then captured on the membrane by the pre-coated anti-human IgM antibody, forming a burgundy colored band on test 1 region, indicating a L.donovani IgM positive test result.

The L.donovani IgG, if present in the specimen, will bind to the Leishmania conjugates. The immunocomplex is then captured by the pre-coated reagents on the membrane, forming a burgundy colored band on test 2 region, indicating a L.donovani IgG positive test result.

Neither of the T band shown suggests a negative result.

Background Information

Visceral leishmaniosis, or Kala-azar, is a disseminated infection caused by several subspecies of L.donovani. The disease is estimated by the World Health Organization to affect approximately 12 million people from 88 countries. It is transmitted to humans by bites of the Phlebotomus sandflies, which acquire infection from feeding on infected animals.

Serological detection of anti-L.donovani IgM is found to be an excellent marker for the acute Visceral leishmaniasis. Recently, the adoption of L. donovani specific protein in the serological test method has improved the sensitivity and specificity dramatically.

Leishmania IgG/IgM blood Test Cassette

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1 LEISH 471 Leishmania IgG/IgM Rapid Test serum/plasma 25 Bulk: 1600 PCS
Boxed: 40 Boxes
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