Leptospirosis IgG/IgM Rapid Test Cassette

Leptospirosis IgG/IgM Rapid Test Cassette

Leptospirosis IgG/IgM Rapid Test Cassette is a lateral flow immunoassay for the simultaneous detection and differentiation of IgG and IgM antibodies to Leptospira interrogans (L.interrogans) in human serum, plasma or whole blood. This rapid Leptospirosis blood test kit is a simple serological test that utilizes antigens from L.interrogans, and detects IgG and IgM antibodies to the microorganisms of L.Interrogans simultaneously.

This Leptospirosis test kit can be performed by untrained or minimally skilled personnel, without cumbersome laboratory equipment within 15 minutes, which makes this test kits very suitable to be used in Point of Care Test (POCT) environment. This Leptospirosis rapid test kit is intended to be used as a blood screening test device, and as an aid in the diagnosis of infection with L.interrogans. Any reactive specimen with the Leptospirosis IgG/IgM Rapid Test must be confirmed with alternative testing methods.

Principle

Leptospirosis IgG/IgM Rapid Test cassette consists of: 1) a burgundy colored conjugate pad containing recombinant L. interrogans antigens conjugated with colloid gold (Leptospira conjugates) and rabbit IgG-gold conjugates, 2) a nitrocellulose membrane strip containing two test bands: one band is pre-coated with monoclonal anti-human IgM for the detection of anti-L.. interrogans IgM, the other band is pre-coated with reagents for the detection of anti-L. interrogans IgG, and the C band is pre-coated with goat anti rabbit IgG.

When an adequate volume of test specimen is dispensed into the sample well of the cassette, the specimen migrates by capillary action across the cassette. IgM antibody to L. interrogans, if present in the specimen, will bind to the Leptospira conjugates. The immunocomplex is then captured on the membrane by the pre-coated anti-human IgM antibody, forming a burgundy colored band, indicating the assay result is L. interrogans IgM positive.

IgG to L. interrogans, if present in the specimen, will bind to the Leptospira conjugates. The immunocomplex is then captured by the pre-coated reagents on the membrane, forming a burgundy colored 2 band, indicating the assay is L. interrogans IgG positive.

Absence of any of the test bands suggests a negative result.

Background Information

Leptospirosis occurs worldwide, and is a common mild to severe health problem for humans and animals, particularly in areas with a hot and humid climate. The natural reservoirs for leptospirosis are rodents as well as a large variety of domesticated mammals. L. interrogans is usually transmitted to humans through contact with infected animal urine, either directly or in water. It invades directly through broken skin and can replicate in the liver and kidneys. Human infection is caused by L. interrogans, the pathogenic member of the genus of Leptospira1,2. The infection is spread via urine from the host animal.

After infection, leptospires are present in the blood until they are cleared after 4 to 7 days following the production of anti-L.interrogans antibodies, initially the IgM class, then the IgG class. Serological detection of anti- L.interrogans antibodies is a common diagnostic method used in the diagnosis of Leptospirosis.

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Leptospirosis IgG/IgM blood Test Cassette

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1 LEPT 481 Leptospirosis IgG/IgM Rapid Test Cassette serum/WB/plasma 25 Bulk: 1600 PCS
Boxed: 40 Boxes
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