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Group-A Rotavirus Test Kits

Rotavirus antigen rapid test cassette, and rotavirus IgG and IgM dot assay rapid test kits are used in the diagnosis of rotavirus diarrhea, and IgG test can also check the immuno status of the human beings.

Group-A Rotavirus Rapid Test Cassette

Group-A Rotavirus Test Kits

Atlas Link Technology Co., Ltd supplies three Rotavirus rapid test kits: rotavirus antigen rapid test cassette, and rotavirus IgG and IgM dot assay rapid test kits, which are all immunoassay kits used as an aid in the diagnosis of rotavirus diarrhea, and IgG test can also check the immuno status of the human beings.

Rotavirus-A Antigen cassette is a rapid testing device based on the gold colloidal solid phase double antibody sandwich immunochromatography principle, to detect group-A specific proteins, including the major inner capsid protein from the Group A rotavirus, in human stool specimens. While the Rotavirus-A IgG Dot assay and Rotavirus-A IgM Dot assay are testing devices based on the dot immunoassay filtration assay principle, intended to be used to detect the IgG and IgM antibodies specific to rotavirus in human serum.

Epidemic of Rotavirus

Rotavirus belongs to the viral family Reoviridae. Three serotypes of the viruses can infect humans, with rotavirus-A accounts for more than 90% of rotavirus gastroenteritis in humans.

Rotavirus is transmitted by the fecal-oral route. The faeces of an infected person can contain more than 10 trillion infectious particles per gram; while 10–100 are enough to transmit the infection. The virus can live a long time in the natural environment, and the usual sanitary measures can't kill the virus effectively. The infection can reoccur during the life time, and most of the subsequent infections are asymptomatic. But the asymptomatic patients can transmit infectious particles. These characters make the rotavirus very epidemic all over the world, both in the developing and developed countries.

Rotavirus Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
RoV 931 Rotavirus AG Test Cassette human faeces 20 Bulk: 1500 PCS
Boxed: 40 Boxes
RoV 9312 Rotavirus IgM Dot Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
RoV 9322 Rotavirus IgG Dot Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
How to Use

Rotavirus AG Test Cassette

General Information

(IVD Kit »-» Digestive Track Disease -» One Step Lateral Flow Immunochromatographic Assay)

Catalog No.:RoV 931
Description:Rotavirus AG Test Cassette
Category:Digestive Track Disease
Style:Card
Unit:PCS
Specimen:human faeces
No. of Step:One Step
Reading Time:10 Minutes
Cut Off:
Specification:4.0 mm
Quan. or Qual.:Qualitative
Sensitivity:94%
Specificity:98.3%
Method:Lateral Flow Double Antibody Sandwich

Packing Specification

Tests per Kit:20 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:40 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1500 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):14.5 KG
GW per CTN (Bulk):14.5 KG

Materials Provided in the Test Kit

  • Test Cassette with desiccant in a single pouch;
  • Sample collection tube with feces sampling stick and sample buffer;
  • Package Insert.

Specimen

Faeces Preparation

Collect feces into a clean, dry specimen collection container.

Unscrew the cap of the specimen collection tube, and randomly poke the specimen collection stick fixed in the cap into the fecal specimen on at least 3 different sites. Do not scoop the fecal specimen.

Tighten the cap of the specimen collection tube, and shake the tube vigorously to mix the specimen and the extraction buffer.

The mixed liquid is the specimen that can be tested immediately.

human faeces Storage Condition

Best results will be obtained if the assay is performed within 6 hours after collection.

Specimen prepared in the specimen collection tube may be stored for 6 months at -20°C.

Let the specimen come to room temperature naturally before testing the refrigerated specimens.

Testing Principle of Lateral Flow Double Antibody Sandwich

GICA Double Antibody Sandwich Method Illustration

In the double antibody sandwich lateral flow chromatographic rapid test, two antibodies are used to detect viral protein or antigen.

In the test band region, antibody specific to the target antigen or protein is immobilized. In the end of the membrane strip where the samples are added, another antibody conjugated with colloidal gold particles is embedded.

During the testing process, specimen from the patient is added to the end of the membrane strip. The antibody conjugate particles will resolve into the specimen, where the antibody conjugate will react and bound to the target protein or viral antigen, if present.

This antigen-antibody conjugate complex then moves along the strip to the testing band region, under capillary effect. The immobilized antibody in the test line region then bound to another binding site on the antigen complex, and the antibody-antigen-antibody complex is fixed on test band region.

When the complex congregates, the highly concentrated colloidal gold particles will develop some red or pink color, indicate a positive result.

If there is no target protein or antigen in the specimen, the colloidal gold particles will not congregate and no color will develop, indicating a negative result.

Information | Packing | Specimen | Principle

Rotavirus IgM Dot Assay

General Information

(IVD Kit »-» Digestive Track Disease -» Dot Immuno Gold Filtration Assay)

Catalog No.:RoV 9312
Description:Rotavirus IgM Dot Assay
Category:Digestive Track Disease
Style:DOT Card
Unit:PCS
Specimen:serum
No. of Step:Two Step
Reading Time:3 Minutes
Cut Off:
Specification:25T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:DGFIA Capture

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:36 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1200 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • Filtration assay device;
  • Washing buffer Gold colloidal Conjugate solution;
  • Disposable dropper for sample transfer;
  • Instruction for use.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of DGFIA Capture

DGFIA Capture Method Illustration

For the detection of IgM-class antibodies, capture technique has its advantage in that it can reduce the interference from the IgG class, thus increase the specificity of the test kit.

In a capture dot filtration test kit, antibodies directed to human IgM antibody (anti-i chain) are pre-coated in the test region on the millipore filtering membrane. When specimen is added, the IgM, if present, will be captured by this secondary antibody, anti-IgM.

After washing away the specimen, especially the IgG antibody, the antigen conjugated with colloidal gold particle will be added. If the IgM antibody was captured in the prior step, the IgM will bind to the antigen conjugate. When the colloidal gold particles congregate, a red or pink color will develop, indicating a positive result.

If no IgM in the specimen, this complex will not develop, the antigen conjugated colloidal gold particles will not congregate, and no color will develop in the test region, indicating a negative result.

Information | Packing | Specimen | Principle

Rotavirus IgG Dot Assay

General Information

(IVD Kit »-» Digestive Track Disease -» Dot Immuno Gold Filtration Assay)

Catalog No.:RoV 9322
Description:Rotavirus IgG Dot Assay
Category:Digestive Track Disease
Style:DOT Card
Unit:PCS
Specimen:serum
No. of Step:Two Step
Reading Time:3 Minutes
Cut Off:
Specification:25T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:DGFIA indirect

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:36 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1200 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • Filtration assay device;
  • Washing buffer Gold colloidal Conjugate solution;
  • Disposable dropper for sample transfer;
  • Instruction for use.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of DGFIA indirect

DGFIA Indirect Method Illustration

With indirect Dot filtration test kit, the millipore filtering membrane is pre-coated with purified or recombinant viral antigens. The detecting conjugate reagent is stored in a vial, whose main component is rabbit anti human IgG (anti-IgG) antibodies, which is conjugated with colloidal gold particles.

During the first step, sample is added to the sample well in the test cassette. The target IgG antibodies, if present, will be captured by the pre-coated antigens.

The wells are washed to remove unbound serum proteins. Then, the colloidal gold conjugate is added. If the target IgG antibodies have been captured in the first step, the IgG antibody will react with the added secondary antibody, anti-IgG conjugate, and red color will develop, indicating a positive result.

Information | Packing | Specimen | Principle