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Salmonella Typhi IgG/IgM Test

Salmonella typhoid IgG/IgM rapid test cassette is solid phase immunochromatographic assay, used in the diagnosis of Salmonella Typhoid Fever.

Typhoid Fever Test Cassette

Intended Use

Salmonella typhoid IgG/IgM rapid test cassette is solid phase immunochromatographic assay for the rapid, qualitative and differential detection of IgG and IgM antibodies to Salmonella typhi in human serum, plasma or whole blood, as an aid in the diagnosis of Typhoid Fever. This test provides only a preliminary testing result. More specific alternative diagnosis method must be used when positive result is observed, in order to obtain a confirmation of Salmonella typhi infection.

Introduction to S.Typhi Test Cassette

There are three pre-coated lines on the strip of Salmonella typhi Test: "G" for the detection of Salmonella typhi IgG antibody, "M" for IgM, and "C" for inner quality control. A purple "G" and/or "M" lines can be observed visually in the result window, if there are enough IgG and/or IgM antibodies to salmonella typhi in the tested specimen, indicating a positive result.

S.Typhi Rapid Test is Helpful

Although Widal test and culture methods are regarded as confirmatory diagnostic methods, they take long time, and it may become later to start antibiotic measures to treat the disease when waiting for the testing results. So, usually, if tested positive with a salmonella typhi rapid testing method, especially if the patient has a contact history with confirmed patient of Typhoid fever, therapeutic trial with chloramphenicol can be taken during this waiting time for the testing result of culture method. In this way, the Salmonella typhi IgG/IgM Test cassette is helpful in the treatment of the typhoid fever.

Typhoid Fever

Typhoid fever is a serious illness caused by a bacterium called Salmonella typhi. Typhoid fever is characterized by a slowly progressive fever as high as 40 centigrade, profuse sweating and gastroenteritis. The World Health Organization identifies typhoid fever as a serious public health problem.

About 10% of untreated patients may shed infectious bacteria in their stool up to 3 months after onset of symptoms and 2%-5% may become permanent shedders, who are called chronic carriers. People can be infected by eating or drinking contaminated food or water or by contacting with patients of Typhoid fever. Common sanitary measures can effectively control the transfer of Salmonella typhi.

Salmonella Typhi Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
SMNL 941 Salmonella Typhi Test Card serum/WB/plasma 25 Bulk: 1500 PCS
Boxed: 40 Boxes
How to Use

Salmonella Typhi Test Card

General Information

(IVD Kit »-» Digestive Track Disease -» One Step Lateral Flow Immunochromatographic Assay)

Catalog No.:SMNL 941
Description:Salmonella Typhi Test Card
Category:Digestive Track Disease
No. of Step:One Step
Reading Time:15-30 Minutes
Cut Off:
Specification:3.0 mm
Quan. or Qual.:Qualitative
Method:Lateral Flow Capture

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:40 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1500 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):14.5 KG
GW per CTN (Bulk):16.0 KG

Materials Provided in the Test Kit

  • Test strip in individual foil pouched with desiccant;
  • 1 bottle of assay buffer;
  • 1 ul Disposable specimen loop;
  • disposable sample tubes;
  • Leaflet of Instruction for use.


Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

Whole Blood Collection

Whole blood can be collected with blood collection tube with venipuncture into blood collection tube (containing EDTA, citrate or heparin).

Or, by lancet lancing. Clean the area to be lanced with an alcohol swab. Squeeze the end of the fingertip and pierce with a sterile lancet. Wipe away the first drop of blood with sterile gauze or cotton. Take a dropper provided, while gently squeezing the dropper tube, immerse the open end in the blood drop and then gently release the pressure to draw blood into the dropper.

Plasma Collection

Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.

Separate the plasma by centrifugation.

Carefully withdraw the plasma into a new pre-labeled tube.

serum/WB/plasma Storage Condition

Test specimens as soon as possible after collecting.

The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times.

Prior to testing, bring frozen specimens to room temperature slowly and mix gently.

Specimens containing visible particulate matter should be clarified by centrifugation before testing.

Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Lateral Flow Capture

GICA Capture Method Illustration

For the detection of IgM-class antibodies, in order to avoid the interference from the IgG class, capture assays technology is usually the best choice.

In capture lateral flow test kit, a secondary antibody, usually antibody directed to human IgM (anti-human IgM), is immobilized in the test band region on the strip membrane. And monoclonal viral antigen or protein conjugated with colloidal gold particle is embedded in the end of membrane strip.

During the testing process, the sample is added to the end of the membrane strip, the embedded antigen conjugate will resolve into the sample, where the target IgM antibody, if present, will react with and bound to the antigen conjugated with colloidal gold particles.

When the IgM-antigen complex moves to the test band region under the capillary effect, the IgM antibody will be captured by the immobilized anti-human IgM antibody. When the colloidal gold particles congregate in the test band region, a red or pink color will develop, indicating a positive result.

If there is no IgM antibody in the specimen, the antigen conjugated with colloidal gold particle can’t be captured by the anti-human IgM secondary antibody, and no color will develop, indicating a negative result.

Information | Packing | Specimen | Principle