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V.Cholera O1 Antigen Rapid Test

Vibrio Cholera O1 (VCO1) rapid test cassette is used to qualitatively detect the vibrio cholera O1 Inaba and ogawa in human feces, rectal swab or environment water specimens.

Vibrio Cholera Test Kit

Intended Use

One step Vibrio Cholera O1 (VCO1) rapid test cassette is a chromatographic immunoassay for the qualitative detection of vibrio cholera O1 Inaba and vibrio cholera O1 ogawa in human feces, rectal swab specimens or environment water. This assay provides a visual, qualitative result. Clinical expertise and professional judgment should be sought to further evaluate the positive testing results of the test cassette.

V.Cholera O1 Antigen Test Brief

Monoclonal antibodies to V. cholerae O-antigen are used in the test device as both capturer and detector materials. The VCO1 rapid test is very sensitive to detect as low concentration of V. cholera O1 of 104 CFU/ml.

The test device has test line 'T' and control line 'C' on the membrane. The control line is used for procedural control. Control line should always appear so as to prove the test procedure is performed properly and the test reagents of control are working. A pink T line will be visible in the result window if enough V.cholerae O1 antigen is present in the sample, indicating a positive result.

V.Cholera and Epidemic Cholera

V.Cholera O1 is a gram-negative pathogen to cause cholera epidemics, which is widely acknowledged as one of the most important pathogen causing gastrointestinal disorders.

It is generally transmitted to human via contaminated water and food. Because of the clinical and epidemiological severity, it is critical to determine as quickly as possible the presence of v. cholerae in clinical specimens, water and food, so that appropriate monitoring and effective preventive measures can be undertaken by public health authorities.

Salmonella Typhi Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
VCO1 951 Vibrio Cholera O1 Rapid Test Feces & Water 25 Bulk: 1600 PCS
Boxed: 40 Boxes
How to Use

Vibrio Cholera O1 Rapid Test

General Information

(IVD Kit »-» Digestive Track Disease -» One Step Lateral Flow Immunochromatographic Assay)

Catalog No.:VCO1 951
Description:Vibrio Cholera O1 Rapid Test
Category:Digestive Track Disease
Specimen:Feces & Water
No. of Step:One Step
Reading Time:15 Minutes
Cut Off:10^4 CFU/ml
Specification:4.0 mm
Quan. or Qual.:Qualitative
Method:Lateral Flow Double Antibody Sandwich

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:40 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1600 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):14.0 KG
GW per CTN (Bulk):16.5 KG

Materials Provided in the Test Kit

  • Test cassette in single foil pouch with desiccant;
  • Disposable sample dropper;
  • Instruction leaflet.


Feces Collection

Collect a random feces sample in a clean, dry container.

Insert the stick or dropper into the feces to get about 0.5g or 0.3ml feces sample.

Add feces sample to the vial of Sample Buffer A, and mix the feces sample with Sample Buffer A thoroughly.

The supernatant will be used for the test.

Environment Water Collection

Collect environment water sample with a clean and dry container, which is ready for the assay immediately.

Feces & Water Storage Condition

Test specimens as soon as possible after collecting.

The specimens can be stored up to 3 days at 2-8°C. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times.

Prior to testing, bring frozen specimens to room temperature slowly.

Testing Principle of Lateral Flow Double Antibody Sandwich

GICA Double Antibody Sandwich Method Illustration

In the double antibody sandwich lateral flow chromatographic rapid test, two antibodies are used to detect viral protein or antigen.

In the test band region, antibody specific to the target antigen or protein is immobilized. In the end of the membrane strip where the samples are added, another antibody conjugated with colloidal gold particles is embedded.

During the testing process, specimen from the patient is added to the end of the membrane strip. The antibody conjugate particles will resolve into the specimen, where the antibody conjugate will react and bound to the target protein or viral antigen, if present.

This antigen-antibody conjugate complex then moves along the strip to the testing band region, under capillary effect. The immobilized antibody in the test line region then bound to another binding site on the antigen complex, and the antibody-antigen-antibody complex is fixed on test band region.

When the complex congregates, the highly concentrated colloidal gold particles will develop some red or pink color, indicate a positive result.

If there is no target protein or antigen in the specimen, the colloidal gold particles will not congregate and no color will develop, indicating a negative result.

Information | Packing | Specimen | Principle