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HCV Rapid and ELISA Kits

Atlas Link Technology Co., Ltd supplies one-step anti-HCV rapid test kits and anti-HCV ELISA Kit.

anti-HCV Test cassette

HCV Test Kits

Atlas Link Technology Co., Ltd supplies one-step anti-HCV rapid test kits and anti-HCV ELISA Kit. All the immunoassay test kits are based on the principle of double antigen sandwich immunoassay, which use purified recombinant HCV antigens to identify hepatitis-C antibody with high sensitivity and specificity. And these hepatitis C kits are widely used as an aid in the diagnosis of Hepatitis-C infection.

Meanings of HCV Testing Result

With these HCV antibody test kits, if positive results are observed, this may mean any of the following:

  • the testee has a short-term infection and will get better on their own (15%);
  • the testee has a long-term infection, and is at risk for developing cirrhosis or liver cancer later in life;
  • the infection has resolved (the person was infected in the past but is no longer infected);
  • the test was incorrect (a false positive result).
So when these screening test kits shows positive result, more advanced technology are recommended to be performed under the administration of a physician.

Hepatitis-C Infection

Hepatitis C is a liver disease caused by the hepatitis C virus. Most infected persons develop chronic, long-term Hepatitis C and are at risk for cirrhosis (scarring of the liver) and liver failure. Hepatitis C accounts for nearly 8,000-10,000 deaths each year, and is regarded as among the most serious disease of the six detectable hepatitis disease types. This means that screening test should be performed with those who are vulnerable to hepatitis-C infection.

Hepatitis C Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
HCV 271 HCV Antibody Test Strip serum 100 Bulk: 6000 PCS
Boxed: 48 Boxes
HCV 272 Anti-HCV Test Cassette serum 25 Bulk: 1600 PCS
Boxed: 40 Boxes
HCV 274 Anti-HCV WB Test Cassette serum/WB 25 Bulk: 1600 PCS
Boxed: 40 Boxes
HCV 2714 Anti-HCV ELISA Kit serum/plasma 96 Boxed: 40 Kits
How to Use

HCV Antibody Test Strip

General Information

(IVD Kit »-» Hepatitis Disease -» One Step Lateral Flow Immunochromatographic Assay)

Catalog No.:HCV 271
Description:HCV Antibody Test Strip
Category:Hepatitis Disease
Style:Dipstick
Unit:PCS
Specimen:serum
No. of Step:One Step
Reading Time:10 Minutes
Cut Off:
Specification:3.0 mm
Quan. or Qual.:Qualitative
Sensitivity:97.9%
Specificity:98.4%
Method:Lateral Flow Double Antigen Sandwich

Packing Specification

Tests per Kit:100 Tests
Box Dimension:21.0 * 12.5 * 5.5 CM
Kits per CTN:48 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):6000 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):16.0 KG

Materials Provided in the Test Kit

  • Lateral Flow immunochromatographic assay (GICA) Strip;
  • Instruction leaflet;
  • Disposable urine cup (optional).

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Lateral Flow Double Antigen Sandwich

GICA Double Antigen Sandwich Method Illustration

Double antigen sandwich technology is commonly used to detect total antibody, which include IgM, IgG and IgA antibodies, which all can react and bound to specific antigen. In the lateral flow double antigen sandwich chromatographic rapid test, recombinant or purified antigens are used to capture and detect the total antibodies specifically.

In the test band region, purified or recombinant antigens or proteins are immobilized. In the end of the membrane strip where the samples are supposed to be added, another antigen conjugated with colloidal gold particles is embedded.

During the testing process, specimen from the patient is added to the end of the membrane strip. The antigen conjugate particles resolve into the specimen, where the antigen conjugate will react and bound to the target antibodies, if present.

This antibody-antigen conjugate complex then moves along the strip to the testing band region, under capillary effect. Where the immobilized antigen captures the complex, and the antigen-antibody-antigen-colloidal gold complex is fixed on test band region. When the complex congregates, the highly concentrated colloidal gold particles will develop some red or pink color, indicate a positive result.

If there is no target antibody in the specimen, the colloidal gold particles will not congregate on the test band region, and no color will develop, indicating a negative result.

Information | Packing | Specimen | Principle

Anti-HCV Test Cassette

General Information

(IVD Kit »-» Hepatitis Disease -» One Step Lateral Flow Immunochromatographic Assay)

Catalog No.:HCV 272
Description:Anti-HCV Test Cassette
Category:Hepatitis Disease
Style:Card
Unit:PCS
Specimen:serum
No. of Step:One Step
Reading Time:10 Minutes
Cut Off:
Specification:4.0 mm
Quan. or Qual.:Qualitative
Sensitivity:97.9%
Specificity:98.4%
Method:Lateral Flow Double Antigen Sandwich

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:40 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1600 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):14.0 KG
GW per CTN (Bulk):16.5 KG

Materials Provided in the Test Kit

  • Test cassette in single foil pouch with desiccant;
  • Disposable sample dropper;
  • Instruction leaflet.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Lateral Flow Double Antigen Sandwich

GICA Double Antigen Sandwich Method Illustration

Double antigen sandwich technology is commonly used to detect total antibody, which include IgM, IgG and IgA antibodies, which all can react and bound to specific antigen. In the lateral flow double antigen sandwich chromatographic rapid test, recombinant or purified antigens are used to capture and detect the total antibodies specifically.

In the test band region, purified or recombinant antigens or proteins are immobilized. In the end of the membrane strip where the samples are supposed to be added, another antigen conjugated with colloidal gold particles is embedded.

During the testing process, specimen from the patient is added to the end of the membrane strip. The antigen conjugate particles resolve into the specimen, where the antigen conjugate will react and bound to the target antibodies, if present.

This antibody-antigen conjugate complex then moves along the strip to the testing band region, under capillary effect. Where the immobilized antigen captures the complex, and the antigen-antibody-antigen-colloidal gold complex is fixed on test band region. When the complex congregates, the highly concentrated colloidal gold particles will develop some red or pink color, indicate a positive result.

If there is no target antibody in the specimen, the colloidal gold particles will not congregate on the test band region, and no color will develop, indicating a negative result.

Information | Packing | Specimen | Principle

Anti-HCV WB Test Cassette

General Information

(IVD Kit »-» Hepatitis Disease -» One Step Lateral Flow Immunochromatographic Assay)

Catalog No.:HCV 274
Description:Anti-HCV WB Test Cassette
Category:Hepatitis Disease
Style:Card
Unit:PCS
Specimen:serum/WB
No. of Step:One Step
Reading Time:5-10 Minutes
Cut Off:
Specification:4.0 mm
Quan. or Qual.:Qualitative
Sensitivity:98.5%
Specificity:99.2%
Method:Lateral Flow Double Antigen Sandwich

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:40 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1600 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):14.0 KG
GW per CTN (Bulk):16.5 KG

Materials Provided in the Test Kit

  • Test cassette in single foil pouch with desiccant;
  • Disposable sample dropper;
  • Instruction leaflet.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

Whole Blood Collection

Whole blood can be collected with blood collection tube with venipuncture into blood collection tube (containing EDTA, citrate or heparin).

Or, by lancet lancing. Clean the area to be lanced with an alcohol swab. Squeeze the end of the fingertip and pierce with a sterile lancet. Wipe away the first drop of blood with sterile gauze or cotton. Take a dropper provided, while gently squeezing the dropper tube, immerse the open end in the blood drop and then gently release the pressure to draw blood into the dropper.

serum/WB Storage Condition

Test specimens as soon as possible after collecting.

The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times.

Prior to testing, bring frozen specimens to room temperature slowly and mix gently.

Specimens containing visible particulate matter should be clarified by centrifugation before testing.

Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Lateral Flow Double Antigen Sandwich

GICA Double Antigen Sandwich Method Illustration

Double antigen sandwich technology is commonly used to detect total antibody, which include IgM, IgG and IgA antibodies, which all can react and bound to specific antigen. In the lateral flow double antigen sandwich chromatographic rapid test, recombinant or purified antigens are used to capture and detect the total antibodies specifically.

In the test band region, purified or recombinant antigens or proteins are immobilized. In the end of the membrane strip where the samples are supposed to be added, another antigen conjugated with colloidal gold particles is embedded.

During the testing process, specimen from the patient is added to the end of the membrane strip. The antigen conjugate particles resolve into the specimen, where the antigen conjugate will react and bound to the target antibodies, if present.

This antibody-antigen conjugate complex then moves along the strip to the testing band region, under capillary effect. Where the immobilized antigen captures the complex, and the antigen-antibody-antigen-colloidal gold complex is fixed on test band region. When the complex congregates, the highly concentrated colloidal gold particles will develop some red or pink color, indicate a positive result.

If there is no target antibody in the specimen, the colloidal gold particles will not congregate on the test band region, and no color will develop, indicating a negative result.

Information | Packing | Specimen | Principle

Anti-HCV ELISA Kit

General Information

(IVD Kit »-» Hepatitis Disease -» Enzyme Linked ImmunoadSorbent Assay)

Catalog No.:HCV 2714
Description:Anti-HCV ELISA Kit
Category:Hepatitis Disease
Style:Microwell
Unit:Box
Specimen:serum/plasma
No. of Step:Two Step
Reading Time:30-30-15 Minutes
Cut Off:
Specification:48T/96T/480T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:Indirect ELISA

Packing Specification

Tests per Kit:96 Tests
Box Dimension:13.5 * 9.0 * 8.0 CM
Kits per CTN:40 Kits
Carton Dimension:59 * 45 * 38 CM
Tests per CTN (BULK):40 Tests
Carton Volume:0.100 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • MICROWELL PLATE: 1 plate;
  • NEGATIVE CONTROL: 1 vial;
  • POSITIVE CONTROL SERUM: 1 vial;
  • SPECIMEN DILUENT: 1 vial;
  • HRP-CONJUGATE REAGENT: 1 vial;
  • WASH BUFFER: 1 bottle;
  • CHROMOGEN SOLUTION A: 1 vial;
  • CHROMOGEN SOLUTION B: 1 vial;
  • STOP SOLUTION: 1 vial;
  • PLASTIC SEALABLE BAG: 1 piece;
  • CARDBOARD PLATE COVER;
  • PACKAGE INSERTS: 1 copy.

Specimen

Plasma Collection

Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.

Separate the plasma by centrifugation.

Carefully withdraw the plasma into a new pre-labeled tube.

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum/plasma Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Indirect ELISA

ELISA Indirect Method Illustration

With indirect ELISA kit, polystyrene microwell strips are pre-coated with purified or recombinant viral antigens.

The antibody detection is achieved in two steps. During the first incubation step, the target antibodies, if present, will be bound to the solid phase pre-coated antigens.

The wells are washed to remove unbound serum proteins, and rabbit anti-human IgG antibodies (anti-IgG) conjugated to horseradish peroxidase (HRP) are added. During the second incubation step, these HRP-conjugated antibodies will be bound to any antigen-antibody complexes previously formed.

Then the unbound HRP-conjugate is then removed by washing. Chromogen solutions containing Tetramethyl benzidine (TMB) and urea peroxide are added to the wells. In presence of the antigen-antibody-anti-IgG (HRP) immuno complex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue colored product which turns blue color turns yellow after stopping the reaction with sulfuric acid, indicating a positive result.

Wells containing samples negative for the target antibodies remain colorless.

Information | Packing | Specimen | Principle