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RSV Dot Assay Kits

Respiratory syncytial virus IgG and IgM Dot assay kits are used as an aid in the diagnosis of RSV infection.

RSV IgM Dot Assay Kit

Intended Use

RSV Dot Assay kits, including RSV IgM Dot Assay and RSV IgG Dot Assay, are a series of rapid test kits based on Gold Immuno-filtration Assay (GIFA) principle, which are used to detect the IgM and IgG antibodies specific to Respiratory syncytial virus (RSV) in human serum specimen, and are intended to be used as an aid in the diagnosis of RSV infection.

Clinical Value of RSV IgM testing

RSV IgG testing results in neonates must be interpreted with caution, because maternal IgG antibody can be transferred passively from the mother to the fetus before delivery, and it will present in the infants' blood for some time. On the other hand, RSV IgM assay results are generally a more useful indicator of RSV infection in children, especially when below 6 months of age. RSV immunity wanes over time, so people can be infected multiple times during life time; no vaccine at present against RSV.

RSV Infection

Respiratory syncytial virus (RSV) is a negative-sense, single-stranded RNA virus, and belongs to the family of Paramyxoviridae. RSV is the major agent of infection in the respiratory tract of children under two years, causing bronchiolitis, pneumonia, trachea-bronchitis and otitis. And it is regarded the first common cause of lower respiratory tract infection to children. And to the immunocompromised patients and premature infants, the RSV infection may cause death. Treatment is limited to supportive care, including oxygen. In temperate climates, RSV is epidemic during the winter. In tropical regions, infection is most common during the rainy season.

Respiratory Syncytial Virus (RSV) Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
RSV 8712 RSV IgM Dot Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
RSV 8722 RSV IgG Dot Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
How to Use

RSV IgM Dot Assay

General Information

(IVD Kit »-» Respiratory Tract Disease -» Dot Immuno Gold Filtration Assay)

Catalog No.:RSV 8712
Description:RSV IgM Dot Assay
Category:Respiratory Tract Disease
Style:DOT Card
Unit:PCS
Specimen:serum
No. of Step:Two Step
Reading Time:3 Minutes
Cut Off:
Specification:25T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:DGFIA Capture

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:36 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1200 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • Filtration assay device;
  • Washing buffer Gold colloidal Conjugate solution;
  • Disposable dropper for sample transfer;
  • Instruction for use.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of DGFIA Capture

DGFIA Capture Method Illustration

For the detection of IgM-class antibodies, capture technique has its advantage in that it can reduce the interference from the IgG class, thus increase the specificity of the test kit.

In a capture dot filtration test kit, antibodies directed to human IgM antibody (anti-i chain) are pre-coated in the test region on the millipore filtering membrane. When specimen is added, the IgM, if present, will be captured by this secondary antibody, anti-IgM.

After washing away the specimen, especially the IgG antibody, the antigen conjugated with colloidal gold particle will be added. If the IgM antibody was captured in the prior step, the IgM will bind to the antigen conjugate. When the colloidal gold particles congregate, a red or pink color will develop, indicating a positive result.

If no IgM in the specimen, this complex will not develop, the antigen conjugated colloidal gold particles will not congregate, and no color will develop in the test region, indicating a negative result.

Information | Packing | Specimen | Principle

RSV IgG Dot Assay

General Information

(IVD Kit »-» Respiratory Tract Disease -» Dot Immuno Gold Filtration Assay)

Catalog No.:RSV 8722
Description:RSV IgG Dot Assay
Category:Respiratory Tract Disease
Style:DOT Card
Unit:PCS
Specimen:serum
No. of Step:Two Step
Reading Time:3 Minutes
Cut Off:
Specification:25T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:DGFIA indirect

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:36 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1200 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • Filtration assay device;
  • Washing buffer Gold colloidal Conjugate solution;
  • Disposable dropper for sample transfer;
  • Instruction for use.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of DGFIA indirect

DGFIA Indirect Method Illustration

With indirect Dot filtration test kit, the millipore filtering membrane is pre-coated with purified or recombinant viral antigens. The detecting conjugate reagent is stored in a vial, whose main component is rabbit anti human IgG (anti-IgG) antibodies, which is conjugated with colloidal gold particles.

During the first step, sample is added to the sample well in the test cassette. The target IgG antibodies, if present, will be captured by the pre-coated antigens.

The wells are washed to remove unbound serum proteins. Then, the colloidal gold conjugate is added. If the target IgG antibodies have been captured in the first step, the IgG antibody will react with the added secondary antibody, anti-IgG conjugate, and red color will develop, indicating a positive result.

Information | Packing | Specimen | Principle