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Measles IgG & IgM Dot Assay Kits

Measles IgG Dot assay Kit and Measles IgM Dot assay Kit are immunoassay kits, used an aid in the diagnosis of Measles infection.

Measles IGG & Measles IGM POCT Dot Assay Kits

Intended Use

Measles IgG Dot assay Kit and Measles IgM Dot assay Kit are immunoassay kits based on the Dot Immuno Gold Filtration Assay (DIGFA) principle, which are intended to be used to detect respectively the IgG and IgM antibody to Morbilli virus in human serum or plasma specimen, as an aid in the diagnosis of Measles infection.

Why Measles Antibody Test Matter

The laboratory diagnosis of IgG and IgM antibodies to measles virus can be of great help in the diagnosis process. When the measles IgM antibody is detected in a patient's blood, it is a strong indicator of measles infection; while a positive result of Measles IgG test result may indicate the past infection, or the immunity status to this disease.

Testing Principle

In these measles antibody test kits, purified measles antigen is used as capturer, which is pre-coated on the nitrocellulose membrane of the dot assay cassette. A second antibody, i.e. anti-human IgG or IgM antibodies labeled with colloidal gold particle is used as detector. When the target IgG or IgM antibody presents in the specimen, the antigen-antibody-secondary antibody complex will form in the testing spot. With the accumulation of colloidal gold particles which is colored, some visual dot will develop in the test region, indicating a positive result.

Measles

Measles, also known as rubeola or morbilli, is a highly infectious respiratory disease. Studies show that 90% of people without immunity will be infected if they shares living space with an infected person. Measles is spread through respiration contact with fluids from an infected person's nose and mouth, either directly or through aerosol droplets transmission.

There is only one measles virus serotype, and the antigenicity is the same, so people who had suffered from measles generally has the immunity to it all the life. Measles is an endemic disease. In populations that have not been exposed to measles, exposure to measles as a new disease can be devastating. According to WHO, measles is a leading cause of vaccine-preventable childhood mortality.

Measles Virus Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
MV 8512 Measles Virus IgM Dot Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
MV 8522 Measles Virus IgG Dot Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
How to Use

Measles Virus IgM Dot Assay

General Information

(IVD Kit »-» Respiratory Tract Disease -» Dot Immuno Gold Filtration Assay)

Catalog No.:MV 8512
Description:Measles Virus IgM Dot Assay
Category:Respiratory Tract Disease
Style:DOT Card
Unit:PCS
Specimen:serum
No. of Step:Two Step
Reading Time:3 Minutes
Cut Off:
Specification:25T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:DGFIA Capture

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:36 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1200 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • Filtration assay device;
  • Washing buffer Gold colloidal Conjugate solution;
  • Disposable dropper for sample transfer;
  • Instruction for use.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of DGFIA Capture

DGFIA Capture Method Illustration

For the detection of IgM-class antibodies, capture technique has its advantage in that it can reduce the interference from the IgG class, thus increase the specificity of the test kit.

In a capture dot filtration test kit, antibodies directed to human IgM antibody (anti-i chain) are pre-coated in the test region on the millipore filtering membrane. When specimen is added, the IgM, if present, will be captured by this secondary antibody, anti-IgM.

After washing away the specimen, especially the IgG antibody, the antigen conjugated with colloidal gold particle will be added. If the IgM antibody was captured in the prior step, the IgM will bind to the antigen conjugate. When the colloidal gold particles congregate, a red or pink color will develop, indicating a positive result.

If no IgM in the specimen, this complex will not develop, the antigen conjugated colloidal gold particles will not congregate, and no color will develop in the test region, indicating a negative result.

Information | Packing | Specimen | Principle

Measles Virus IgG Dot Assay

General Information

(IVD Kit »-» Respiratory Tract Disease -» Dot Immuno Gold Filtration Assay)

Catalog No.:MV 8522
Description:Measles Virus IgG Dot Assay
Category:Respiratory Tract Disease
Style:DOT Card
Unit:PCS
Specimen:serum
No. of Step:Two Step
Reading Time:3 Minutes
Cut Off:
Specification:25T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:DGFIA indirect

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:36 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1200 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • Filtration assay device;
  • Washing buffer Gold colloidal Conjugate solution;
  • Disposable dropper for sample transfer;
  • Instruction for use.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of DGFIA indirect

DGFIA Indirect Method Illustration

With indirect Dot filtration test kit, the millipore filtering membrane is pre-coated with purified or recombinant viral antigens. The detecting conjugate reagent is stored in a vial, whose main component is rabbit anti human IgG (anti-IgG) antibodies, which is conjugated with colloidal gold particles.

During the first step, sample is added to the sample well in the test cassette. The target IgG antibodies, if present, will be captured by the pre-coated antigens.

The wells are washed to remove unbound serum proteins. Then, the colloidal gold conjugate is added. If the target IgG antibodies have been captured in the first step, the IgG antibody will react with the added secondary antibody, anti-IgG conjugate, and red color will develop, indicating a positive result.

Information | Packing | Specimen | Principle