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anti-Trophoblast IgG DOT Assay Kit

We supply anti-Trophoblast IgG DOT Assay rapid test kit, which are used as an aid in the diagnosis of infertility.

Anti-Trophoblast IgG DOT Assay Kit

Intended Use

Anti-trophoblast antibody Dot assay (ATA) IgG Rapid test kit is a rapid test kit based on the Gold Immuno-filtration Assay (GIFA) principle, and it is intended to be used to detect the trophoblast specific IgG antibody in human serum specimen, and used as an aid in the diagnosis of infertility.

ATA Testing Principle

In this ATA test kit, purified trophoblast antigen is used as marker, and secondary antibodies, i.e. mouse anti-human IgG antibody conjugated with colloidal gold is used as detector. During the assay test, the IgG antibody specific to Trophoblast, if presents in the tested specimen, antigen+anti-ATr+anti-human-colloidal gold complex will develop in the test spot region, and colored spot will appear, indicating a positive result. If the specimen doesn't contain IgG antibody to Trophoblast, the colloidal gold particle will not be bound on the test region, and no color develops there, indicating a negative result.

Why ATA Testing Matter

In a normal pregnancy, trophoblast cells aid the implantation of the fertilized egg into the uterine wall. When anti-trophoblast antibodies present, the action of the trophoblast cells may be suppressed, and may affect the implantation of the fetus, thus making it hard to become pregnant. As a result, Anti-trophoblast antibodies are usually used as an indicator of infertility.

ATA IgG Antibody and Infertility

Infertility primarily refers to the biological inability of a person to contribute to conception. Many reasons have been suggested, and the present of anti-trophoblast antibodies is one of them.

Trophoblasts are cells forming the outer layer of a blastocyst, which provide nutrients to the embryo and develop into a large part of the placenta. They are formed during the first stage of pregnancy and are the first cells to differentiate from the fertilized egg.

Anti-Trophoblast Antibody (ATA) Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
ATA I622 Trophoblast IgG Dot Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
How to Use

Trophoblast IgG Dot Assay

General Information

(IVD Kit »-» Immunological Infertility -» Dot Immuno Gold Filtration Assay)

Catalog No.:ATA I622
Description:Trophoblast IgG Dot Assay
Category:Immunological Infertility
Style:DOT Card
Unit:PCS
Specimen:serum
No. of Step:Two Step
Reading Time:3 Minutes
Cut Off:
Specification:25T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:DGFIA indirect

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:36 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1200 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • Filtration assay device;
  • Washing buffer Gold colloidal Conjugate solution;
  • Disposable dropper for sample transfer;
  • Instruction for use.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of DGFIA indirect

DGFIA Indirect Method Illustration

With indirect Dot filtration test kit, the millipore filtering membrane is pre-coated with purified or recombinant viral antigens. The detecting conjugate reagent is stored in a vial, whose main component is rabbit anti human IgG (anti-IgG) antibodies, which is conjugated with colloidal gold particles.

During the first step, sample is added to the sample well in the test cassette. The target IgG antibodies, if present, will be captured by the pre-coated antigens.

The wells are washed to remove unbound serum proteins. Then, the colloidal gold conjugate is added. If the target IgG antibodies have been captured in the first step, the IgG antibody will react with the added secondary antibody, anti-IgG conjugate, and red color will develop, indicating a positive result.

Information | Packing | Specimen | Principle