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anti-hCG IgG DOT Assay Kit

Anti-hCG IgG test kit is used as an clinical indicator in the diagnosis of infertility among women.

anti-HCG dot assay kit

Intended Use

Anti-hCG IgG DOT Assay Kit is a rapid test kit, intended to qualitatively detect the IgG antibody specific to Human chorionic gonadotropin (hCG) in human serum specimen, and is used as an clinical indicator in the diagnosis of infertility among women.

anti-HCG Testing Principle

In this Anti-HCG IgG DOT test, nitrocellulose membrane is pre-coated with purified hCG. During the assay, the anti-HCG IgG antibody, if present in the specimen, will be captured by the hCG. In the subsequent procedure, the reagent of anti-human monoclonal IgG antibody (anti-human) conjugated with colloidal gold is added. The secondary antibody will be captured by the bound anti-hCG, and a pink color will develop, indicating a positive result.

Why anti-HCG influences Pregnancy

Human chorionic gonadotropin (hCG) plays an essential role in the implantation of the blastocyst, which help the fetus to cancel rejection by suppressing the immune system. But when anti-HCG concentration is high, these antibodies will affect the function of hCG, thus reduce the chance of pregnancy. This effect of embryo Interception of implantation by anti-hCG antibodies is proved by various studies.

anti-HCG and Infertility

Studies shows sexually active women rarely become pregnant, in whose blood the anti-hCG antibody concentration usually remains above 50 ng/mL. Also, studies show the levels of estradiol (E2) and progesterone (P) in anti-HCG positive group were significantly lower than that in anti-HCG negative group. These study results proves that combined detection of anti-HCG, estradiol and progesterone were of important clinical value in diagnosis of infertile women.

anti-hCG Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
AhCG I322 Anti-hCG IgG Dot Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
How to Use

Anti-hCG IgG Dot Assay

General Information

(IVD Kit »-» Immunological Infertility -» Dot Immuno Gold Filtration Assay)

Catalog No.:AhCG I322
Description:Anti-hCG IgG Dot Assay
Category:Immunological Infertility
Style:DOT Card
No. of Step:Two Step
Reading Time:3 Minutes
Cut Off:
Quan. or Qual.:Qualitative
Method:DGFIA indirect

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:36 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1200 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • Filtration assay device;
  • Washing buffer Gold colloidal Conjugate solution;
  • Disposable dropper for sample transfer;
  • Instruction for use.


Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of DGFIA indirect

DGFIA Indirect Method Illustration

With indirect Dot filtration test kit, the millipore filtering membrane is pre-coated with purified or recombinant viral antigens. The detecting conjugate reagent is stored in a vial, whose main component is rabbit anti human IgG (anti-IgG) antibodies, which is conjugated with colloidal gold particles.

During the first step, sample is added to the sample well in the test cassette. The target IgG antibodies, if present, will be captured by the pre-coated antigens.

The wells are washed to remove unbound serum proteins. Then, the colloidal gold conjugate is added. If the target IgG antibodies have been captured in the first step, the IgG antibody will react with the added secondary antibody, anti-IgG conjugate, and red color will develop, indicating a positive result.

Information | Packing | Specimen | Principle