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ASO IgG Dot Filtration Assay Kit

ASO DOT dot filtration rapid assay kit is a quick test kit for the detection of Anti Streptolysin-O antibody in human serum.

ASO IgG DOT Assay Kit

Intended Use

Anti-Streptolysin O (ASO) DOT Assay is used to detect the IgG antibodies specific to anti-Streptolysin O in human serum sample, and is intended to be used as an aid in the diagnosis of current versus past infections with group A streptococci; it also can be used to help to diagnose rheumatic Arthritis.

Testing Principle

In the anti-Streptolysin O (ASO) DOT test cassette, nitrocellulose membrane is pre-coated with Monoclonal Streptolysin O antigen. During the assay, the anti-Streptolysin O IgG antibody, if present in the serum specimen, will be captured by the pre-coated ASO antigen. In the subsequent procedure, the reagent of anti-human monoclonal IgG antibody (anti-human) conjugated with colloidal gold is added. This secondary antibody will be captured by the ASO IgG antibody bound to the membrane in previous step, and a colored dot will develop, indicating a positive result.

ASO Antibody and Rheumatoid Arthritis

When Lancefield group A streptococci infect human body, they produce many enzymes, including oxygen labile streptolysin-O and oxygen stable streptolysin-S. Both the enzymes are involved in incurring of hemolysis, especially in beta-hemolysis. When human body is infected with streptococci, it produces antibodies against these two antigens. Anti-streptolysin O (ASO) is the antibody produced against streptolysin O antigen. An elevated or rising level indicate a past or present infection of streptococci. Until now, ASO is one of the first bacterial markers used for diagnosis and follow up of rheumatism or scarlet fever.

Anti-Streptolysin O (ASO) Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
ASO R222 Streptolysin-O IgG Dot Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
How to Use

Streptolysin-O IgG Dot Assay

General Information

(IVD Kit »-» Rheumarthritis -» Dot Immuno Gold Filtration Assay)

Catalog No.:ASO R222
Description:Streptolysin-O IgG Dot Assay
Category:Rheumarthritis
Style:DOT Card
Unit:PCS
Specimen:serum
No. of Step:Two Step
Reading Time:3 Minutes
Cut Off:
Specification:25T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:DGFIA indirect

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:36 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1200 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • Filtration assay device;
  • Washing buffer Gold colloidal Conjugate solution;
  • Disposable dropper for sample transfer;
  • Instruction for use.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of DGFIA indirect

DGFIA Indirect Method Illustration

With indirect Dot filtration test kit, the millipore filtering membrane is pre-coated with purified or recombinant viral antigens. The detecting conjugate reagent is stored in a vial, whose main component is rabbit anti human IgG (anti-IgG) antibodies, which is conjugated with colloidal gold particles.

During the first step, sample is added to the sample well in the test cassette. The target IgG antibodies, if present, will be captured by the pre-coated antigens.

The wells are washed to remove unbound serum proteins. Then, the colloidal gold conjugate is added. If the target IgG antibodies have been captured in the first step, the IgG antibody will react with the added secondary antibody, anti-IgG conjugate, and red color will develop, indicating a positive result.

Information | Packing | Specimen | Principle