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CCP IgG Antibody Dot Filtration Assay Kit

CCP IgG Dot test kit is a rapid immunoassay as an aid in the diagnosis of Rheumatoid Arthritis illness.

Anti-CCP IgG DOT Assay Kit

Intended Use

Anti-CCP IgG antibody Dot Assay kit is an quick point-of-care test kit for the detection of IgG antibody specific to Cyclic Citrullinated Peptide (CCP) in human serum sample, which is intended to be used as a tool for the diagnosis of Rheumatoid Arthritis.

Why CCP IgG Test Important

The earlier the diagnosis of rheumatoid arthritis, the sooner targeted treatments can be initiated, thus severe damages to the joints can be avoided or controlled. Even for those patients diagnosed as rheumatoid arthritis by physical examination, clinical symptoms and other blood test result, if the CCP IgG blood test result is negative, the concentration of this autoantibody may increase in later stages of the disease. So, for the CCP IgM negative patients, the test for CCP antibody will be performed regularly during the treatment of the disease.

CCP Antibody and Rheumatoid Arthritis

Cyclic Citrullinated Peptide Antibody (CCP Ab) is an autoantibody present in the blood of about 70% of the rheumatoid arthritis patients. Comparing with other dot filtration blood assay kits for the diagnosis of rheumatoid arthritis, a positive CCP IgG antibody test is considered highly specific to rheumatoid arthritis.

Anti Cyclic Citrulline Polypeptide Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
CCP R322 Anti-CCP IgG DOA Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
How to Use

Anti-CCP IgG DOA Assay

General Information

(IVD Kit »-» Rheumarthritis -» Dot Immuno Gold Filtration Assay)

Catalog No.:CCP R322
Description:Anti-CCP IgG DOA Assay
Style:DOT Card
No. of Step:Two Step
Reading Time:3 Minutes
Cut Off:
Quan. or Qual.:Qualitative
Method:DGFIA indirect

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:36 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1200 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • Filtration assay device;
  • Washing buffer Gold colloidal Conjugate solution;
  • Disposable dropper for sample transfer;
  • Instruction for use.


Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of DGFIA indirect

DGFIA Indirect Method Illustration

With indirect Dot filtration test kit, the millipore filtering membrane is pre-coated with purified or recombinant viral antigens. The detecting conjugate reagent is stored in a vial, whose main component is rabbit anti human IgG (anti-IgG) antibodies, which is conjugated with colloidal gold particles.

During the first step, sample is added to the sample well in the test cassette. The target IgG antibodies, if present, will be captured by the pre-coated antigens.

The wells are washed to remove unbound serum proteins. Then, the colloidal gold conjugate is added. If the target IgG antibodies have been captured in the first step, the IgG antibody will react with the added secondary antibody, anti-IgG conjugate, and red color will develop, indicating a positive result.

Information | Packing | Specimen | Principle