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dsDNA IgG Dot Filtration Assay Kit

dsDNA IgG Dot Assay kit is a one-step rapid test kit, which is used for the detection of IgG antibody specific to double stranded DNA in human serum.

dsDNA IgG DOT rapid Test

Intended Use

dsDNA IgG Dot Assay kit is a one-step rapid test kit, which is used for the detection of IgG antibody specific to double stranded DNA (ds-DNA) in human serum, as an important clinical indicator in the diagnosis of the systemic Lupus Erythematosus (SLE) as well as Rheumatoid Arthritis illness.

dsDNA Testing Principle

In this dsDNA IgG rapid test kit, purified ds-DNA antigen is coated on the surface of membrane. During testing, the ds-DNA IgG antibody, if present, will be captured by the dsDNA antigen and be fixed on the membrane. Then after washing away the serum specimen to clear the unbound proteins, the reagent containing antibody to human IgG (anti-IgG) antibody conjugated with colloidal gold particles is added, the bound ds-DNA antibody will capture the anti-IgG-colloidal gold particles. The accumulation of the colloidal gold particle on the testing spot region will show a colored spot, indicating a positive result of dsDNA IgG antibody.

dsDNA and SLE Disease

Antibodies reacting with native double-stranded DNA are regarded as being specific to systemic lupus erythematosus (SLE) and can be detected in approximately 50-80% of the SLE patients. Antibodies against dsDNA are found during active phases of SLE, their serum concentration tend to positively correlate with the severity of the disease. Thus, detection of these autoantibodies is important for the diagnosis and clinical monitoring of the disease and consequently has been established as 1 of the 11 American College of Rheumatology (ACR) criteria for the diagnosis of SLE.

Systemic Lupus Erythematosus (SLE)

The systemic lupus erythematosus (SLE) is a systemic autoimmune disease that can affect any part of the body, including the heart, joints, skin, lungs, blood vessels, liver, kidneys, and nervous system. Under hypersensitivity reaction, the immune system attacks its own body's cells and tissue, resulting in inflammation and tissue damage. The course of the disease is unpredictable. SLE can be treated by addressing its symptoms, mainly with cyclophosphamide, corticosteroids and immune-suppressants; there is currently no cure. SLE can be fatal, although with recent medical advances, fatalities are becoming increasingly rare.

Double-stranded DNA (dsDNA) Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
dsDNA R422 Double Stranded DNA IgG DOT Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
How to Use

Double Stranded DNA IgG DOT Assay

General Information

(IVD Kit »-» Rheumarthritis -» Dot Immuno Gold Filtration Assay)

Catalog No.:dsDNA R422
Description:Double Stranded DNA IgG DOT Assay
Category:Rheumarthritis
Style:DOT Card
Unit:PCS
Specimen:serum
No. of Step:Two Step
Reading Time:3 Minutes
Cut Off:
Specification:25T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Method:DGFIA indirect

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:36 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1200 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • Filtration assay device;
  • Washing buffer Gold colloidal Conjugate solution;
  • Disposable dropper for sample transfer;
  • Instruction for use.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of DGFIA indirect

DGFIA Indirect Method Illustration

With indirect Dot filtration test kit, the millipore filtering membrane is pre-coated with purified or recombinant viral antigens. The detecting conjugate reagent is stored in a vial, whose main component is rabbit anti human IgG (anti-IgG) antibodies, which is conjugated with colloidal gold particles.

During the first step, sample is added to the sample well in the test cassette. The target IgG antibodies, if present, will be captured by the pre-coated antigens.

The wells are washed to remove unbound serum proteins. Then, the colloidal gold conjugate is added. If the target IgG antibodies have been captured in the first step, the IgG antibody will react with the added secondary antibody, anti-IgG conjugate, and red color will develop, indicating a positive result.

Information | Packing | Specimen | Principle