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ssDNA IgG Dot Filtration Assay Kit

ssDNA IgG Dot test kit is intended to be used as an aid in the diagnosis of Rheumatoid Arthritis.

ssDNA IgG Dot Assay Cassette

Intended Use

ssDNA IgG Dot filtration test kit is an immunoassay based on the principle of Gold Filtration immune Assay principle, intended for the qualitative detection of IgG antibody specific to Single-Strand DNA (ss-DNA) in human serum, and intended to be used as an aid in the diagnosis of Rheumatoid Arthritis.


Antibodies to single-stranded DNA (ssDNA) also have a correlation with the SLE cased. The ssDNA is found with a frequency of up to 87% during acute phases and 43% during inactive phases in patients with SLE. As anti-ssDNA antibodies are found with a frequency of up to 50% in patients with a drug-induced SLE. The determination of antibodies against ssDNA, together with other test such as dsDNA testing, may be helpful for the differential diagnosis.

ssDNA, not a Specific Indicator of SLE

ssDNA is not specific for SLE as they may be found in other diseases, including infectious mononucleosis (40%), autoimmune hepatitis (58%), acute myeloid leukemia (89%), acute lymphatic leukemia, chronic myeloid leukemia (60%) and juvenile rheumatoid arthritis (35-50%).

Rheumatoid Factor (RF) Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
ssDNA R722 ssDNA IgG Dot Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
How to Use

ssDNA IgG Dot Assay

General Information

(IVD Kit »-» Rheumarthritis -» Dot Immuno Gold Filtration Assay)

Catalog No.:ssDNA R722
Description:ssDNA IgG Dot Assay
Style:DOT Card
No. of Step:Two Step
Reading Time:3 Minutes
Cut Off:
Quan. or Qual.:Qualitative
Method:DGFIA indirect

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:36 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1200 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):15.0 KG
GW per CTN (Bulk):15.0 KG

Materials Provided in the Test Kit

  • Filtration assay device;
  • Washing buffer Gold colloidal Conjugate solution;
  • Disposable dropper for sample transfer;
  • Instruction for use.


Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of DGFIA indirect

DGFIA Indirect Method Illustration

With indirect Dot filtration test kit, the millipore filtering membrane is pre-coated with purified or recombinant viral antigens. The detecting conjugate reagent is stored in a vial, whose main component is rabbit anti human IgG (anti-IgG) antibodies, which is conjugated with colloidal gold particles.

During the first step, sample is added to the sample well in the test cassette. The target IgG antibodies, if present, will be captured by the pre-coated antigens.

The wells are washed to remove unbound serum proteins. Then, the colloidal gold conjugate is added. If the target IgG antibodies have been captured in the first step, the IgG antibody will react with the added secondary antibody, anti-IgG conjugate, and red color will develop, indicating a positive result.

Information | Packing | Specimen | Principle