Thyroxine (T4) ELISA Kit

Anti-Zona Pellucida IgG DOT Assay Kit

Thyroxine (T4) ELISA kit is an enzyme-linked immunosorbent assay kit, designed for the quantitative determination of Thyroxine (T4) concentration in human serum, which is used an important indicator in the diagnosis of Thyroid Disorders.

Principle of T4 ELISA Kit

In the T4 test kits, a certain amount of anti-T4 antibody is pre-coated on microtiter wells. A measured amount of patient serum, and a constant amount of T4 conjugated with horseradish peroxidase (T4-HRP) are added to the microtiter wells. 8-anilino-1-napthalene sulphonate (ANS) is used to displace T4 from proteins to enable the measurement of total circulating T4. During incubation, T4 and conjugated T4 compete for the limited binding sites on the anti-T4 antibody. Then the wells are washed with wash solution to remove the unbound reagents and specimen. The T4 captured by the anti-T4 antibody is proportional to the T4 concentration in the serum specimens; while is inversely related to the captured T4-HRP. This relation can be quantified by comparing to the calibration curve, which can be drawn with the help of the positive controls.

Thyroxine (T4) and the diagnosis of thyroid Disorders

Thyroxine (T4) is the major hormone produced by the thyroid gland. T4 is involved in controlling the rate of metabolic processes in the body and influencing physical development. Thyroxine is a prohormone and a reservoir for the active thyroid hormone triiodothyronine. T4 has a molecular weight of 777 daltons and is synthesized by iodination of tyrosine residues on thyroglobulin. Proteolytic cleavage of follicular thyroglobulin releases T4 into the bloodstream. The normal human adult range of T4 in blood is 4 - 11 mcg/dL. Greater than 99.5% of T4 is reversibly bound to 3 plasma proteins in blood - thyroxine binding globulin (TBG) binds 70%, thyroxine binding pre-albumin (TBPA) binds 20%, and albumin binds 10%. Approximately 0.03% of T4 is in the free, unbound state in blood. The total Thyroxine concentration in serum depends both the free thyroxine and the bound thyroxine.

Diseases affecting thyroid function may present a wide array of symptoms. Measurement of total T4 by immunoassay is the most reliable and convenient screening test available to determine the presence of thyroid disorders in patients. Increased levels of T4 have been found in hyperthyroidism due to Grave’s disease and Plummer’s disease and in acute and subacute thyroiditis. Low levels of T4 have been associated with congenital hypothyroidism, myxedema, chronic thyroiditis (Hashimoto’s disease), and with some genetic abnormalities.

Antinuclear IgG Immunoassay Kits

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1 T4 T414 Total Thyroxine (T4) ELISA Kit serum 96T Boxed: 40 Kits boxes
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