Free Triiodothyronine (f-T3) ELISA Kit

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The f-T3 ELISA test kit is intended for the quantitative determination of free triiodothyronine (f-T3) concentration in human serum, which is an important indicator in the diagnosis of Hyperthyroidism and other related disorders.

Principle of f-T3 ELISA Kit

In this f-T3 assay kit, a certain amount of T3 analog is coated on microtiter wells. A measured amount of patient serum and a constant amount of anti-T3 antibody conjugated with horseradish peroxidase are added to the microtiter wells. During the incubation, T3 analog on microtiter wells and free T3 present in the sample compete for binding to the anti-T3 HRP conjugate. The concentration of free T3 in a patient sample is then determined by interpolation from the standard curve. The Related Light Unit (RLU) is directly proportional to the amount of enzyme present and is inversely related to the amount of unlabeled fT3 in the sample. By reference to a series of fT3 standards assayed in the same way, the concentration of fT3 in the unknown sample is quantified.

Free Triiodothyronine and Hyperthyroidism

Triiodothyronine, a thyroid hormone, affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate. It circulates in blood almost completely bound (>99.5%) to plasma proteins. However, only the free (unbound) portion of triiodothyronine is believed to be responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alter, the total triiodothyronine level changes while the free triiodothyronine concentration remains constant. Thus, measurements of free triiodothyronine concentrations correlate more reliably with clinical status than total triiodothyronine levels. The f-T3 ELISA test kit provides the technician with optimum sensitivity in a direct determination of free T3.

f-T3 ELISA Kit

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1 fT3 T114 Free Triiodothyronine (f-T3) ELISA Kit serum 96T Boxed: 40 Kits boxes
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