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AFP Rapid Test Kits

AFP test strip and cassette are used as an aid in the medical diagnosis of primary hepatocellular carcinomas, testicular teratocarcinoma and neural tube defects.

AFP Serum Test strip and cassette

Intended Use

AFP Rapid Test kits are a rapid direct binding immunoassay medical test for the qualitative detection of the tumor marker Alpha-fetoprotein in human serum or plasma specimen. One-Step AFP cancer test kits are used as an aid in the medical diagnosis of primary hepatocellular carcinomas, testicular teratocarcinoma and neural tube defects. Monoclonal and polyclonal antibodies are employed to identity AFP specifically.

Why AFP Testing Matter

An elevated AFP level has been termed by promotion as "the single most discriminating laboratory test indicative of malignant disease." For this character, Alpha-fetoprotein rapid test kits are used as an aid in the diagnosis of primary hepatocellular carcinomas, testicular teratocarcinomas and neural tube defects. Atlas Link Technology Co., Ltd would like to provide quality AFP serum test cassette and AFP serum test strip at competitive prices.

AFP as A Tumor Marker

AFP is a glycoprotein, which belongs to the alpha-globulin family of human plasma proteins. AFP is located primarily in the fetal liver and can be found in fetal and maternal blood and in amniotic fluid. In adults, the normal concentration is only about 10ng/ml. In the serum of patients suffering primary hepatomas and yolk sac derived tumors, the AFP concentration often rises, and the determination of AFP is a useful aid in diagnosis and management of hepatocellular carcinoma.

Alpha Feto-Protein (AFP) Test Kits: Principle, Packing...

Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
AFP 511 AFP Rapid Test Strip serum 100 Bulk: 6000 PCS
Boxed: 48 Boxes
AFP 512 AFP Rapid Test Cassette serum 25 Bulk: 1600 PCS
Boxed: 40 Boxes
How to Use

AFP Rapid Test Strip

General Information

(IVD Kit »-» Tumor Marker -» One Step Lateral Flow Immunochromatographic Assay)

Catalog No.:AFP 511
Description:AFP Rapid Test Strip
Category:Tumor Marker
Style:Dipstick
Unit:PCS
Specimen:serum
No. of Step:One Step
Reading Time:15-20 Minutes
Cut Off:25 ng/ml
Specification:3.0 mm
Quan. or Qual.:Qualitative
Sensitivity:99%
Specificity:99.5%
Method:Lateral Flow Double Antibody Sandwich

Packing Specification

Tests per Kit:100 Tests
Box Dimension:21.0 * 12.5 * 5.5 CM
Kits per CTN:48 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):6000 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):14.0 KG
GW per CTN (Bulk):16.5 KG

Materials Provided in the Test Kit

  • Test Strip sealed in single pouch;
  • Assay Buffer 4 vials;
  • Instruction for Use.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Lateral Flow Double Antibody Sandwich

GICA Double Antibody Sandwich Method Illustration

In the double antibody sandwich lateral flow chromatographic rapid test, two antibodies are used to detect viral protein or antigen.

In the test band region, antibody specific to the target antigen or protein is immobilized. In the end of the membrane strip where the samples are added, another antibody conjugated with colloidal gold particles is embedded.

During the testing process, specimen from the patient is added to the end of the membrane strip. The antibody conjugate particles will resolve into the specimen, where the antibody conjugate will react and bound to the target protein or viral antigen, if present.

This antigen-antibody conjugate complex then moves along the strip to the testing band region, under capillary effect. The immobilized antibody in the test line region then bound to another binding site on the antigen complex, and the antibody-antigen-antibody complex is fixed on test band region.

When the complex congregates, the highly concentrated colloidal gold particles will develop some red or pink color, indicate a positive result.

If there is no target protein or antigen in the specimen, the colloidal gold particles will not congregate and no color will develop, indicating a negative result.

Information | Packing | Specimen | Principle

AFP Rapid Test Cassette

General Information

(IVD Kit »-» Tumor Marker -» One Step Lateral Flow Immunochromatographic Assay)

Catalog No.:AFP 512
Description:AFP Rapid Test Cassette
Category:Tumor Marker
Style:Card
Unit:PCS
Specimen:serum
No. of Step:One Step
Reading Time:15-20 Minutes
Cut Off:25 ng/ml
Specification:4.0 mm
Quan. or Qual.:Qualitative
Sensitivity:99%
Specificity:99.5%
Method:Lateral Flow Double Antibody Sandwich

Packing Specification

Tests per Kit:25 Tests
Box Dimension:21.0 * 12.5 * 6.5 CM
Kits per CTN:40 Kits
Carton Dimension:53 * 45 * 36 CM
Tests per CTN (BULK):1600 Tests
Carton Volume:0.085 CBM
GW per CTN (Boxed):14.0 KG
GW per CTN (Bulk):16.5 KG

Materials Provided in the Test Kit

  • Test cassette in single foil pouch with desiccant;
  • Disposable sample dropper;
  • Specimen Diluents;
  • Instruction leaflet.

Specimen

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Testing Principle of Lateral Flow Double Antibody Sandwich

GICA Double Antibody Sandwich Method Illustration

In the double antibody sandwich lateral flow chromatographic rapid test, two antibodies are used to detect viral protein or antigen.

In the test band region, antibody specific to the target antigen or protein is immobilized. In the end of the membrane strip where the samples are added, another antibody conjugated with colloidal gold particles is embedded.

During the testing process, specimen from the patient is added to the end of the membrane strip. The antibody conjugate particles will resolve into the specimen, where the antibody conjugate will react and bound to the target protein or viral antigen, if present.

This antigen-antibody conjugate complex then moves along the strip to the testing band region, under capillary effect. The immobilized antibody in the test line region then bound to another binding site on the antigen complex, and the antibody-antigen-antibody complex is fixed on test band region.

When the complex congregates, the highly concentrated colloidal gold particles will develop some red or pink color, indicate a positive result.

If there is no target protein or antigen in the specimen, the colloidal gold particles will not congregate and no color will develop, indicating a negative result.

Information | Packing | Specimen | Principle